FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques

Cerebrospinal fluid (csf) drains via the lymphatic drainage pathway. this lymphatic pathway connects the central nervous system (cns) to the cervical lymph node (cln). as the csf drains to cln via the dural and nasal lymphatics, t cells and antigen presenting cells pass along the channels from the subarachnoid space through the cribriform plate. human immunodeficiency virus (hiv) also egress from the cns along this pathway. as a result, hiv egressing from the cns accumulate within the cln. towards this objective, we analyzed clns isolated from rhesus macaques that were chronically-infected with simian immunodeficiency virus (siv). we detected significant accumulation of siv within the clns. siv virion trapping was observed on follicular dendritic cells (fdcs) localized within the follicular regions of clns. in addition, siv antigens formed immune complexes when fdcs interacted with b cells within the germinal centers. subsequent interaction of these b cells with cd4+ t follicular helper cells (tfhs) resulted in infection of the latter. of note, 73% to 90% of the tfhs cells within clns were positive for siv p27 antigen. as such, it appears that not only do the fdcs retain siv they also transmit them (via b cells) to tfhs within these clns. this interaction results in infection of tfhs in the clns. based on these observations, we infer that fdcs within the clns have a novel role in siv entrapment with implications for viral trafficking.