Two non-steroidal anti-inflammatory drugs,niflumic acid and diclofenac,inhibit the human glutamate transporter EAAT1 through different mechanisms

We investigated the effects of non-steroidal anti-inflammatory drugs on substrate-induced currents of l-glutamate (l-glu) transporter eaat1 expressed in xenopus laevis oocytes. niflumic acid (nfa) and diclofenac inhibited l-glu-induced current through eaat1 in a noncompetitive manner. nfa produced a leftward shift in reversal potential (erev ) of l-glu-induced current and increased current amplitude at the potentials more negative than -100 mv. diclofenac had no effects on erev and inhibited the current amplitude to the same extent at all negative potentials. these results indicate that nfa and diclofenac inhibit the l-glu-induced eaat1 current via different mechanisms. [supplementary methods and figure: available only at http://dx.doi.org/10.1254/ jphs.09260sc]. ©2010 the japanese pharmacological society.