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Sodium-calcium exchangers in rat ameloblasts
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نویسنده
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okumura r. ,shibukawa y. ,muramatsu t. ,hashimoto s. ,nakagawa k.-i. ,tazaki m. ,shimono m.
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منبع
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journal of pharmacological sciences - 2010 - دوره : 112 - شماره : 2 - صفحه:223 -230
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چکیده
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Although the central role of ameloblasts in synthesis and resorption of enamel matrix proteins during amelogenesis is well documented,the ca 2+-transport/extrusion mechanism remains to be fully elucidated. to clarify ca2+-transport in rat ameloblasts,we investigated expression and localization of na+-ca2+ exchanger (ncx) isoforms and the functional characteristics of their ion transporting/pharmacological properties. rt-pcr and immunohistochemical analyses revealed expression of ncx1 and ncx3 in ameloblasts,localized in the apical membrane. in patch-clamp recordings,ca2+ efflux by na+-ca2+ exchange showed dependence on external na+. ca2+ influx by na +-ca2+ exchange,measured by fura-2 fluorescence,showed dependence on extracellular ca2+ concentration,and it was blocked by ncx inhibitors kb-r7943,sea0400,and sn-6. these results showed significant expression of ncx1 and ncx3 in ameloblasts,indicating their involvement in the directional ca2+ extrusion pathway from cells to the enamel mineralizing front. ©2010 the japanese pharmacological society.
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کلیدواژه
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Channel; Enamel; Mineralization; SLC8 gene family; Transporter
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آدرس
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oral health science center,hrc7,tokyo dental college,mihama-ku,chiba 261-8502,japan,department of endodontics,pulp and periapical biology,tokyo dental college,mihama-ku,chiba 261-8502,japan,department of pathology,tokyo dental college,mihama-ku,chiba 261-8502, Japan, oral health science center,hrc7,tokyo dental college,mihama-ku,chiba 261-8502,japan,department of physiology,tokyo dental college,1-2-2 masago,mihama-ku,chiba 261-8502, Japan, oral health science center,hrc7,tokyo dental college,mihama-ku,chiba 261-8502,japan,department of pathology,tokyo dental college,mihama-ku,chiba 261-8502, Japan, oral health science center,hrc7,tokyo dental college,mihama-ku,chiba 261-8502,japan,department of pathology,tokyo dental college,mihama-ku,chiba 261-8502, Japan, department of endodontics,pulp and periapical biology,tokyo dental college,mihama-ku,chiba 261-8502, Japan, oral health science center,hrc7,tokyo dental college,mihama-ku,chiba 261-8502,japan,department of physiology,tokyo dental college,1-2-2 masago,mihama-ku,chiba 261-8502, Japan, department of pathology,tokyo dental college,mihama-ku,chiba 261-8502, Japan
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Authors
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