Direct assessments of the antioxidant effects of the novel collagen peptide on reactive oxygen species using electron spin resonance spectroscopy

In the present study,we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (p-ncp) on reactive oxygen species (ros) such as hydroxyl radical (ho•),superoxide anion radical (o 2 •-),and singlet oxygen (1o2),and the effects on cell viability after ultraviolet ray (uv) irradiation of human fibroblasts. we confirmed,using electron spin resonance,that p-ncp directly inhibited ho• and 1o2. furthermore,addition of p-ncp to fibroblasts inhibited cell death induced by uva (400 - 315 nm) irradiation in a dose-dependent manner. in addition,the antioxidant effect on 1o2 was observed in the peptide fractions rich in gly,pro,hyp,glu,ala,and arg. we found that gly,hyp,glu,and ala directly scavenged 1o2. these results indicated that a peptide sequence including gly,hyp,glu,and ala could play a key role in the antioxidant effects of p-ncp on 1o2. it was suggested that p-ncp can inhibit photo-aging related to ros owing to its antioxidant effects. © the japanese pharmacological society.