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   History of the G protein-coupled receptor (GPCR) assays from traditional to a state-of-the-art biosensor assay  
   
نویسنده miyano k. ,sudo y. ,yokoyama a. ,hisaoka-nakashima k. ,morioka n. ,takebayashi m. ,nakata y. ,higami y. ,uezono y.
منبع journal of pharmacological sciences - 2014 - دوره : 126 - شماره : 4 - صفحه:302 -309
چکیده    The g protein-coupled receptors (gpcrs) form the largest and the most versatile superfamily that share a seven-transmembrane-spanning architecture. gpcr-signaling is involved in vision,taste,olfaction,sympathetic/parasympathetic nervous functions,metabolism,and immune regulation,indicating that gpcrs are extremely important therapeutic targets for various diseases. cellular dielectric spectroscopy (cds) is a novel technology that employs a label-free,real-time and cell-based assay approach for the comprehensive pharmacological evaluation of cells that exogenously or endogenously express gpcrs. among the biosensors that use cds technology,the cellkey™ system not only detects the activation of gpcrs but also distinguishes between signals through different subtypes of the gα protein (gs,gi/o,and gq). in this review,we discuss the traditional assays and then introduce the principles by which the cellkey™ system evaluates gpcr activation,followed by a perspective on the advantages and future prospects of this system. © the japanese pharmacological society.
کلیدواژه CellKey™ system; Cellular dielectric spectroscopy; Electrical impedance-based biosensor; G protein-coupled receptor; GPCR assay method
آدرس division of cancer pathophysiology,national cancer center research institute,5-1-1 tsukiji,chuo-ku,tokyo,104-0045, Japan, division of cancer pathophysiology,national cancer center research institute,5-1-1 tsukiji,chuo-ku,tokyo,104-0045,japan,department of molecular pathology and metabolic disease,faculty of pharmaceutical sciences,tokyo university of science,2641 yamazaki,noda,chiba,278-0022, Japan, division of cancer pathophysiology,national cancer center research institute,5-1-1 tsukiji,chuo-ku,tokyo,104-0045,japan,department of molecular pathology and metabolic disease,faculty of pharmaceutical sciences,tokyo university of science,2641 yamazaki,noda,chiba,278-0022, Japan, department of pharmacology,hiroshima university,graduate school of biomedical sciences,1-2-3 kasumi,minami-ku,hiroshima,734-8553, Japan, department of pharmacology,hiroshima university,graduate school of biomedical sciences,1-2-3 kasumi,minami-ku,hiroshima,734-8553, Japan, division of psychiatry and neuroscience,institute for clinical research,department of psychiatry,3-1 aoyama,kure,737-0023, Japan, department of pharmacology,hiroshima university,graduate school of biomedical sciences,1-2-3 kasumi,minami-ku,hiroshima,734-8553, Japan, department of molecular pathology and metabolic disease,faculty of pharmaceutical sciences,tokyo university of science,2641 yamazaki,noda,chiba,278-0022, Japan, division of cancer pathophysiology,national cancer center research institute,5-1-1 tsukiji,chuo-ku,tokyo,104-0045, Japan
 
     
   
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