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   The water-extracted Ampelopsis brevipedunculata downregulates IL-1β,CCL5,and COX-2 expression via inhibition of PKC-mediated JNK/NF-κB signaling pathways in human monocytic cells  
   
نویسنده le m.q. ,kim m.s. ,song y.s. ,noh w.n. ,chun s.c. ,yoon d.y.
منبع journal of pharmacological sciences - 2014 - دوره : 126 - شماره : 4 - صفحه:359 -369
چکیده    The anti-inflammatory and anti-hepatotoxic effects of ampelopsis brevipedunculata (a.bre) have been well known in folk medicine. an ethanol-extract of a.bre has been reported to inhibit carbon tetrachloric acid induced hepatic injury,suggesting that extracted components from a.bre could potentially treat inflammatory disease. to test this hypothesis,in this study,we extracted polysaccharide components from leaves of a.bre and investigated the anti-inflammatory effects in pma stimulated thp-1 cells. thp-1 cells activated by pma in the presence or absence of a.bre demonstrated that a water-extract of a.bre inhibited the expression of pro-inflammatory cytokine il-1β and chemokine ccl-5 in a dose-dependent manner. in addition,a.bre suppressed production of cyclooxygenase (cox)-2 in thp-1 cells activated by pma. moreover,a.bre markedly down-regulated the expression of p-jnk1/3,whereas it did not inhibit production of the phosphorylated form of p38 and extracellular signal-regulated kinase in thp-1 cells treated by pma. particularly,a.bre inhibited the translocation of transcription factor nf-κb from the cytosol into the nucleus in pma-stimulated thp-1 cells. collectively,our data showed that water-extracted a.bre inhibited the protein kinase c-jnks/nf-κb signaling pathways,resulting in the suppression of il-1β,ccl-5,and cox-2 expression. this study suggests that water extracted a.bre may be a therapeutic agent against inflammatory disease. © the japanese pharmacological society.
کلیدواژه Ampelopsis brevipedunculata; Anti-inflammation; IL-1β; PMA; THP-1
آدرس laboratory of cellular biology and immunobiochemistry,department of bioscience and biotechnology,seoul,143-701, South Korea, laboratory of cellular biology and immunobiochemistry,department of bioscience and biotechnology,seoul,143-701, South Korea, laboratory of cellular biology and immunobiochemistry,department of bioscience and biotechnology,seoul,143-701, South Korea, department of bioresources and food science,konkuk university,seoul,143-701, South Korea, department of bioresources and food science,konkuk university,seoul,143-701, South Korea, laboratory of cellular biology and immunobiochemistry,department of bioscience and biotechnology,seoul,143-701, South Korea
 
     
   
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