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Antiproliferative effect of echinacoside on rat pulmonary artery smooth muscle cells under hypoxia
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نویسنده
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gai x.-y. ,tang f. ,ma j. ,zeng k.-w. ,wang s.-l. ,wang y.-p. ,wuren t.-n. ,lu d.-x. ,zhou y. ,ge r.-l.
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منبع
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journal of pharmacological sciences - 2014 - دوره : 126 - شماره : 2 - صفحه:155 -163
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چکیده
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The main purpose of this study is to evaluate the effect of echinacoside (ech) on hypoxia-induced proliferation of rat pulmonary artery smooth muscle cells (pasmcs) and the underlying mechanism. pasmcs were incubated under normoxia (nor),hypoxia (hyp),hypoxia + 0.35 mm ech (hyp + ech0.35),or hypoxia + 0.4 mm ech (hyp + ech0.4) for 24 h. cell viability was assessed by mts assays. the morphology of apoptosis was observed by dapi staining,and apoptosis was quantified by flow cytometric analysis. caspase-3 activity was determined by immunohistochemistry and real-time pcr,and the expressions of hif-1α,bax,bcl-2,and fas were determined by real-time pcr. hypoxia induced significant proliferation of pasmcs,which could be inhibited by ech in a concentration-dependent manner. this was associated with apoptosis of pasmcs. z-devd-fmk could partly reduce the suppression effect of ech; protein and gene expression of caspase-3 were significantly higher in the hyp + ech0.4 and hyp + ech0.35 groups. ech significantly increased the expressions of bax and fas,but decreased the expressions of bcl-2 and hif-1α. ech could inhibit hypoxia-induced proliferation of rat pasmcs,which is associated with apoptosis of pasmcs and improvement of hypoxia. ech might be a potential agent for prevention and treatment of hypoxia-induced pah. © the japanese pharmacological society.
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کلیدواژه
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Apoptosis; High altitude; Proliferation of rat pulmonary artery smooth muscle cell (pasmc); Pulmonary arterial hypertension; Tibetan herb
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آدرس
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department of pharmacology,school of life science and biopharmaceutics,shenyang pharmaceutical university,shenyang,liaoning,110016, China, research center for high altitude medicine,medical college of qinghai university,xining,qinghai,810001, China, department of endemic diseases,institute for endemic diseases prevention and control of qinghai province,xining,qinghai,810001, China, state key laboratory of natural and biomimetic drugs,school of pharmaceutical sciences,peking university,beijing,100191, China, research center for high altitude medicine,medical college of qinghai university,xining,qinghai,810001, China, research center for high altitude medicine,medical college of qinghai university,xining,qinghai,810001, China, research center for high altitude medicine,medical college of qinghai university,xining,qinghai,810001, China, research center for high altitude medicine,medical college of qinghai university,xining,qinghai,810001, China, department of pharmacology,school of life science and biopharmaceutics,shenyang pharmaceutical university,shenyang,liaoning,110016,china,research center for high altitude medicine,medical college of qinghai university,xining,qinghai,810001, China, department of pharmacology,school of life science and biopharmaceutics,shenyang pharmaceutical university,shenyang,liaoning,110016,china,research center for high altitude medicine,medical college of qinghai university,xining,qinghai,810001, China
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Authors
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