|
|
|
|
Electron microscopic examination of podosomes induced by phorbol 12,13 dibutyrate on the surface of A7r5 cells
|
|
|
|
|
|
|
|
نویسنده
|
tanaka h. ,wang h.-h. ,thatcher s.e. ,hagiwara h. ,takano-ohmuro h. ,kohama k.
|
|
منبع
|
journal of pharmacological sciences - 2015 - دوره : 128 - شماره : 2 - صفحه:78 -82
|
|
چکیده
|
The role of myosin light chain kinase (mlck) in inducing podosomes was examined by confocal and electron microscopy. removal of myosin from the actin core of podosomes using blebbistatin,a myosin inhibitor,resulted in the formation of smaller podosomes. downregulation of mlck by the transfection of mlck small interfering rna (sirna) led to the failure of podosome formation. however,ml-7,an inhibitor of the kinase activity of mlck,failed to inhibit podosome formation. based on our previous report (thatcher et al. j.pharm.sci. 116 116-127,2011),we outlined the important role of the actin-binding activity of mlck in producing smaller podosomes.
|
|
کلیدواژه
|
Immunoelectron microscopy; Myosin light chain kinase; Podosome
|
|
آدرس
|
department of anatomy,teikyo university school of medicine,2-11-1 kaga,itabashi-ku,tokyo 173-8605, Japan, department of cellular and molecular medicine,university of california san diego,9500 gillman drive 0651,san diego,ca 92093-0651,united states,college of biology,hunan university,yuelushan,no.1 denggao road,changsha,hunan 410082, China, department of nutritional sciences,university of kentucky,900 south limestone,charles t.wethington bldg,lexington,ky 40536-0200, United States, department of anatomy,teikyo university school of medicine,2-11-1 kaga,itabashi-ku,tokyo 173-8605, Japan, research institute of pharmaceutical sciences,musashino university,nishitokyo,tokyo 202-8585, Japan, research institute of pharmaceutical sciences,musashino university,nishitokyo,tokyo 202-8585, Japan
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|