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A new method for measuring osteoclast formation by electrical impedance
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نویسنده
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emori h. ,iwai s. ,ryu k. ,amano h. ,sambe t. ,kobayashi t. ,oguchi t. ,ohura k. ,oguchi k.
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منبع
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journal of pharmacological sciences - 2015 - دوره : 128 - شماره : 2 - صفحه:87 -91
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چکیده
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Osteoclasts are important target cells for osteoporosis treatment. recently,a real-time cell analysis (rtca) system was developed to observe cell morphology and adhesion; however,the use of rtca to study osteoclastogenesis has not been reported. here,we investigated whether osteoclast formation could be monitored in real-time using rtca. the cell index determined via electrical impedance using rtca,and the number of osteoclasts exhibited a significant positive correlation. rtca was useful for determining the effect of (-)-epigallocatechin-3-gallate on the inhibition of bone resorption. we established a new method of measuring osteoclast formation in real-time using rtca. © 2015 production and hosting by elsevier b.v. on behalf of japanese pharmacological society.
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کلیدواژه
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Cell morphology; Osteoclast; Real-time cell analyzing system
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آدرس
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department of pharmacology,showa university school of medicine,1-5-8 hatanodai,shinagawa,tokyo,142-8555,japan,department of orthopaedic surgery,showa university school of medicine,1-5-8 hatanodai,shinagawa-ku,tokyo,142-8555, Japan, department of healthcare and regulatory sciences,showa university school of pharmacy,1-5-8 hatanodai,shinagawa-ku,tokyo,142-8555, Japan, department of pharmacology,showa university school of medicine,1-5-8 hatanodai,shinagawa,tokyo,142-8555, Japan, department of pharmacology,osaka dental university,8-1 kuzuhahanazono-cho,hirakata,osaka,573-1121, Japan, department of pharmacology,showa university school of medicine,1-5-8 hatanodai,shinagawa,tokyo,142-8555, Japan, department of pharmacology,showa university school of medicine,1-5-8 hatanodai,shinagawa,tokyo,142-8555, Japan, department of pharmacology,showa university school of medicine,1-5-8 hatanodai,shinagawa,tokyo,142-8555, Japan, department of pharmacology,osaka dental university,8-1 kuzuhahanazono-cho,hirakata,osaka,573-1121, Japan, department of pharmacology,showa university school of medicine,1-5-8 hatanodai,shinagawa,tokyo,142-8555, Japan
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Authors
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