TC-2559,an α4β2 nicotinic acetylcholine receptor agonist,suppresses the expression of CCL3 and IL-1β through STAT3 inhibition in cultured murine macrophages

The anti-inflammatory properties of tc-2559,an α4β2 nicotinic acetylcholine receptor (nachr) agonist,on cultured murine macrophages was investigated. tc-2559 suppressed the upregulation of cc-chemokine ligand 3 (ccl3) and interleukin-1β (il-1β) following lipopolysaccharide (lps) treatment in j774a.1 cells. tc-2559 inhibited the phosphorylation of signal transducer and activator of transcription 3 (pstat3) but not nuclear factor-κb p65 after lps. blockade of pstat3 by ag490 inhibited the upregulation of ccl3 and il-1β after lps. in conclusion,tc-2559-driven α4β2 nachr signaling suppressed the upregulation of ccl3 and il-1β by inhibiting pstat3 in inflammatory macrophages,resulting in the suppression of neuropathic pain. © 2015 the authors. production and hosting by elsevier b.v. on behalf of japanese pharmacological society. this is an open access article under the cc by-nc-nd license.