Involvement of intracellular cAMP in epirubicin-induced vascular endothelial cell injury

We investigated the involvement of intracellular camp in endothelial cell injury induced by epirubicin. epirubicin-induced decrease in cell viability and increase in caspase-3/7 activity were reversed by a camp analog dibutyryl camp (dbcamp) or an activator of adenylate cyclase forskolin concomitant with a phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. moreover,epirubicin-induced elevation of lipid peroxide levels was attenuated by dbcamp. interestingly,the exposure of epirubicin decreased intracellular camp levels before the onset of epirubicin-induced production of lipid peroxidation. these results suggest that intracellular camp plays an important role in epirubicin-induced endothelial cell injury. © 2016 japanese pharmacological society.