The prostaglandin E2/EP4 receptor/cyclic AMP/T-type Ca2+ channel pathway mediates neuritogenesis in sensory neuron-like ND7/23 cells

We investigated mechanisms for the neuritogenesis caused by prostaglandin e2 (pge2) or intracellular cyclic amp (camp) in sensory neuron-like nd7/23 cells. pge2 caused neuritogenesis,an effect abolished by an ep4 receptor antagonist or inhibitors of adenylyl cyclase (ac) or protein kinase a (pka) and mimicked by the ac activator forskolin,dibutyryl camp (db-camp),and selective activators of pka or epac. nd7/23 cells expressed both cav3.1 and cav3.2 t-type ca2+ channels (t-channels). the neuritogenesis induced by db-camp or pge2 was abolished by t-channel blockers. t-channels were functionally upregulated by db-camp. the pge2/ep4/camp/t-channel pathway thus appears to mediate neuritogenesis in sensory neurons. © 2016 japanese pharmacological society.