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A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
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نویسنده
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lópez-redondo f. ,kurokawa j. ,nomura f. ,kaneko t. ,hamada t. ,furukawa t. ,yasuda k.
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منبع
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journal of pharmacological sciences - 2016 - دوره : 131 - شماره : 2 - صفحه:141 -145
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چکیده
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We investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes,and analyzed action potential parameters by plotting their frequency distributions. in the both cell lines,the distribution analysis revealed that histograms of maximum upstroke velocity showed two subpopulations with similar intersection values. sub-populations with faster maximum upstroke velocity showed significant prolongation of action potential durations by application of e-4031,whereas others did not,which may be partly due to shallower maximum diastolic potentials. we described electrophysiological and pharmacological properties of stem-cell-derived cardiomyocytes in the respective sub-populations,which provides a way to characterize diverse electrical properties of stem-cell-derived cardiomyocytes systematically. © 2016 the authors
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کلیدواژه
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Cardiomyocyte; iPS cells; Patch-clamp
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آدرس
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department of biomedical information,institute of biomaterials and bioengineering,tokyo medical and dental university,tokyo,101-0062, Japan, department of bio-informational pharmacology,medical research institute,tokyo medical and dental university,tokyo,113-8510, Japan, department of biomedical information,institute of biomaterials and bioengineering,tokyo medical and dental university,tokyo,101-0062, Japan, department of frontier bioscience,hosei university,koganei,tokyo,184-8584, Japan, department of biomedical information,institute of biomaterials and bioengineering,tokyo medical and dental university,tokyo,101-0062, Japan, department of bio-informational pharmacology,medical research institute,tokyo medical and dental university,tokyo,113-8510, Japan, department of biomedical information,institute of biomaterials and bioengineering,tokyo medical and dental university,tokyo,101-0062, Japan
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Authors
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