High glucose induces N-linked glycosylation-mediated functional upregulation and overexpression of Cav3.2 T-type calcium channels in neuroendocrine-like differentiated human prostate cancer cells

Given that cav3.2 t-type ca2+ channels were functionally regulated by asparagine (n)-linked glycosylation,we examined effects of high glucose on the function of cav3.2,known to regulate secretory function,in neuroendocrine-like differentiated prostate cancer lncap cells. high glucose accelerated the increased channel function and overexpression of cav3.2 during neuroendocrine differentiation,the former prevented by enzymatic inhibition of n-glycosylation and cleavage of n-glycans. hyperglycemia thus appears to induce n-linked glycosylation-mediated functional upregulation and overexpression of cav3.2 in neuroendocrine-like differentiated prostate cancer cells. © 2017 the authors