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Demeanor of rivaroxaban in activated/inactivated FXa
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نویسنده
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seki k. ,mizuno y. ,sakashita t. ,nakano s. ,tanno j. ,okazaki y. ,muramatsu t. ,nishimura s. ,senbonmatsu t.
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منبع
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journal of pharmacological sciences - 2017 - دوره : 133 - شماره : 3 - صفحه:156 -161
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چکیده
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Activated factor x (fxa) plays an important role in thrombin generation and inflammation. factor x is not converted constitutively to fxa,but only after intrinsic clotting factors are activated and/or cellular injury occurs. although rivaroxaban is one of direct fxa inhibitors,its function in the inactivated coagulation cascade is unclear. in human umbilical vein endothelial cells that natively express protease-activated receptor-1 and -2,high dose rivaroxaban did not alter gene transcripts including pro-inflammatory genes in dna microarray. upon fxa stimulation,the expressions of pro-inflammatory genes such as monocyte chemoattractant protein-1 (mcp-1),intracellular adhesion molecule-1,and interleukin-8 were maximally increased at 4 h after stimulation,and were suppressed by rivaroxaban. to confirm these results,quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (elisa) for mcp-1 were performed. fxa evoked the expression of mcp-1 maximally at 4 h after stimulation,whereas mcp-1 displayed a different temporal activation in elisa. interestingly,rivaroxaban inhibited both time courses of mcp-1 expression. these results suggest that rivaroxaban may not influence gene modulation in the inactivated coagulation state,but can attenuate the endothelial damage evoked by fxa and pro-inflammatory cytokine genes. © 2017 the authors
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کلیدواژه
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Activated factor X; Anticoagulant; Gene modulation; Inflammation; Rivaroxaban
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آدرس
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department of cardiology,saitama medical university,international medical center,saitama, Japan, division of functional genomics & systems medicine,saitama medical university research center for genomic medicine,saitama, Japan, department of cardiology,saitama medical university,international medical center,saitama, Japan, department of cardiology,saitama medical university,international medical center,saitama, Japan, department of cardiology,saitama medical university,international medical center,saitama, Japan, division of functional genomics & systems medicine,saitama medical university research center for genomic medicine,saitama, Japan, department of cardiology,saitama medical university,international medical center,saitama, Japan, department of cardiology,saitama medical university,international medical center,saitama, Japan, department of cardiology,saitama medical university,international medical center,saitama,japan,research administration center,saitama medical university,saitama, Japan
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Authors
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