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Inhibitory effects of pine nodule extract and its component,SJ-2,on acetylcholine-induced catecholamine secretion and synthesis in bovine adrenal medullary cells
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نویسنده
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li x. ,horishita t. ,toyohira y. ,shao h. ,bai j. ,bo h. ,song x. ,ishikane s. ,yoshinaga y. ,satoh n. ,tsutsui m. ,yanagihara n.
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منبع
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journal of pharmacological sciences - 2017 - دوره : 133 - شماره : 4 - صفحه:268 -275
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چکیده
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Extract of pine nodules (matsufushi) formed by bark proliferation on the surface of trees of pinus tabulaeformis or pinus massoniana has been used as an analgesic for joint pain,rheumatism,neuralgia,dysmenorrhea and other complaints in chinese traditional medicine. here we report the effects of matsufushi extract and its components on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. we found that matsufushi extract (0.0003–0.005%) and its component,sj-2 (5-hydroxy-3-methoxy-trans-stilbene) (0.3–100 μm),but not the other three,concentration-dependently inhibited catecholamine secretion induced by acetylcholine,a physiological secretagogue. matsufushi extract (0.0003–0.005%) and sj-2 (0.3–100 μm) also inhibited 45ca2+ influx induced by acetylcholine in a concentration-dependent manner,similar to its effect on catecholamine secretion. they also suppressed 14c-catecholamine synthesis and tyrosine hydroxylase activity induced by acetylcholine. in xenopus oocytes expressing α3β4 nicotinic acetylcholine receptors,matsufushi extract (0.00003–0.001%) and sj-2 (1–100 μm) directly inhibited the current evoked by acetylcholine. the present findings suggest that sj-2,as well as matsufushi extract,inhibits acetylcholine-induced catecholamine secretion and synthesis by suppression of nicotinic acetylcholine receptor-ion channels in bovine adrenal medullary cells. © 2017 the authors
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کلیدواژه
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Adrenal medulla; Catecholamine secretion; Matsufushi; Nicotinic acetylcholine receptor-ion channels; SJ-2
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آدرس
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department of pharmacology,university of occupational and environmental health,school of medicine,1-1,iseigaoka,yahatanishi-ku,kitakyushu,807-8555, Japan, department of anesthesiology,university of occupational and environmental health,school of medicine,1-1,iseigaoka,yahatanishi-ku,kitakyushu, Japan, department of pharmacology,university of occupational and environmental health,school of medicine,1-1,iseigaoka,yahatanishi-ku,kitakyushu,807-8555, Japan, tokujun pharmaceutical research institute,kobe, Japan, tokujun pharmaceutical research institute,kobe, Japan, tokujun pharmaceutical research institute,kobe, Japan, tianjin zhongyi pharmaceutical co. ltd.,tianjin university of traditional chinese medicine,tianjin, China, department of pharmacology,university of occupational and environmental health,school of medicine,1-1,iseigaoka,yahatanishi-ku,kitakyushu,807-8555, Japan, department of pharmacology,university of occupational and environmental health,school of medicine,1-1,iseigaoka,yahatanishi-ku,kitakyushu,807-8555, Japan, shared-use research center,university of occupational and environmental health,school of medicine,1-1,iseigaoka,yahatanishi-ku,kitakyushu, Japan, department of pharmacology,faculty of medicine,university of the ryukyus,nishiharaokinawa, Japan, department of pharmacology,university of occupational and environmental health,school of medicine,1-1,iseigaoka,yahatanishi-ku,kitakyushu,807-8555, Japan
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Authors
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