opposite regulation of pirnas, rrnas and mirnas in the blood after subarachnoid hemorrhage

Multiple classes of small rnas (srnas) are expressed in the blood and are involved in the regulation of pivotal cellular processes. we aimed to elucidate the expression patterns and functional roles of srnas in the systemic response to intracranial aneurysm (ia) rupture. we used next-generation sequencing to analyze the expression of srnas in patients in the acute phase of ia rupture (first 72 h), in the chronic phase (3–15 months), and controls. the patterns of alterations in srna expression were analyzed in the context of clinically relevant information regarding the biological consequences of ia rupture. we identified 542 differentially expressed srnas (108 pirnas, 99 rrnas, 90 mirnas, 43 scrnas, 36 trnas, and 32 snornas) among the studied groups with notable differences in upregulated and downregulated srnas between the groups and srnas categories. pirnas and rrnas showed a substantial decrease in rna abundance that was sustained after ia rupture, whereas mirnas were largely upregulated. downregulated srna genes included pir-31080, pir-57947, 5s rrna, lsu-rrna, and ssu-rrna s. remarkable enrichment in the representation of transcription factor binding sites was revealed in genomic locations of the regulated srna. we found strong overrepresentation of glucocorticoid receptor, retinoid x receptor alpha, and estrogen receptor alpha binding sites at the locations of downregulated pirnas, trnas, and rrnas. this report, although preliminary and largely proof-of-concept, is the first to describe alterations in srnas abundance levels in response to ia rupture in humans. the obtained results indicate novel mechanisms that constitute another level of control of the inflammatory response.