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heterologous prime-boost vaccination against tuberculosis with recombinant sendai virus and dna vaccines
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نویسنده
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hu zhidong ,jiang weimin ,gu ling ,qiao dan ,shu tsugumine ,lowrie douglas b. ,lu shui-hua ,fan xiao-yong
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منبع
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journal of molecular medicine - 2019 - دوره : 97 - شماره : 12 - صفحه:1685 -1694
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چکیده
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In an earlier study, a novel sendai virus–vectored anti-tuberculosis vaccine encoding ag85a and ag85b (sev85ab) was constructed and shown to elicit antigen-specific t cell responses and protection against mycobacterium tuberculosis (mtb) infection in a murine model. in this study, we evaluate whether the immune responses induced by this novel vaccine might be elevated by a recombinant dna vaccine expressing the same antigen in a heterologous prime-boost vaccination strategy. the results showed that both sev85ab prime-dna boost (sev85ab-dna) and dna prime-sev85ab boost (dna-sev85ab) vaccination strategies significantly enhanced the antigen-specific t cell responses induced by the separate vaccines. the sev85ab-dna immunization regimen induced higher levels of recall t cell responses after mtb infection and conferred better immune protection compared with dna-sev85ab or a single immunization. collectively, our study lends strong evidence that a dna vaccine boost might be included in a novel sev85ab immunization strategy designed to enhance the immune protection against mtb.
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کلیدواژه
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tuberculosis ,sendai virus ,dna vaccine ,prime-boost ,t cell responses
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آدرس
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fudan university, shanghai public health clinical center, key laboratory of medical molecular virology of moe/moh, china, fudan university, huashan hospital, departments of infectious diseases, china, fudan university, shanghai public health clinical center, key laboratory of medical molecular virology of moe/moh, china. wenzhou medical university, school of laboratory medicine and life science, china. shanghai emerging and re-emerging institute, tb center, china, shanghai jiaotong university, ruijin hospital (north), china, id pharma, japan, fudan university, shanghai public health clinical center, key laboratory of medical molecular virology of moe/moh, china. shanghai emerging and re-emerging institute, tb center, china, fudan university, shanghai public health clinical center, key laboratory of medical molecular virology of moe/moh, china. wenzhou medical university, school of laboratory medicine and life science, china. shanghai emerging and re-emerging institute, tb center, china, wenzhou medical university, school of laboratory medicine and life science, china. shanghai emerging and re-emerging institute, tb center, china
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Authors
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