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   Ixodes scapularis dystroglycan-like protein promotes Borrelia burgdorferi migration from the gut  
   
نویسنده Coumou Jeroen ,Narasimhan Sukanya ,Trentelman Jos J. ,Wagemakers Alex ,Koetsveld Joris ,Ersoz Jasmin I. ,Oei Anneke ,Fikrig Erol ,Hovius Joppe W.
منبع journal of molecular medicine - 2016 - دوره : 94 - شماره : 3 - صفحه:361 -370
چکیده    The causative agent of lyme borreliosis, borrelia burgdorferi, is transmitted by ixodes ticks. during tick feeding, b. burgdorferi migrates from the tick gut to the salivary glands from where transmission to the host occurs. b. burgdorferi-interacting tick proteins might serve as vaccine targets to thwart b. burgdorferi transmission. a previous screening for b. burgdorferi-interacting ixodes scapularis gut proteins identified an i. scapularis putative dystroglycan protein (iscw015049). here, we describe the iscw015049’s protein structure and its cellular location in the tick gut in relation to b. burgdorferi migration. secondly, in vivo b. burgdorferi–tick attachment murine models were performed to study the role of iscw015049 during b. burgdorferi migration and transmission. in silico analysis confirmed that iscw015049 is similar to dystroglycan and was named i. scapularis dystroglycan-like protein (isdlp). confocal microscopy of gut tissue showed that isdlp is expressed on the surface of gut cells, is upregulated during tick feeding, and is expressed significantly higher in infected ticks compared to uninfected ticks. inhibition of isdlp by rna interference (rnai) resulted in lower b. burgdorferi transmission to mice. in conclusion, we have identified a dystroglycan-like protein in i. scapularis gut that can bind to b. burgdorferi and promotes b. burgdorferi migration from the tick gut.
کلیدواژه Tick-borne diseases ,Lyme borreliosis ,Ixodes scapularis ,Borrelia burgdorferi ,Pathogen transmission ,Vaccination
آدرس University of Amsterdam, The Netherlands, Yale University School of Medicine, Department of Internal Medicine, USA, University of Amsterdam, The Netherlands, University of Amsterdam, The Netherlands, University of Amsterdam, The Netherlands, University of Amsterdam, The Netherlands, University of Amsterdam, Department of Medical Microbiology, The Netherlands, Yale University School of Medicine, Department of Internal Medicine, USA, University of Amsterdam, The Netherlands
 
     
   
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