>
Fa   |   Ar   |   En
   Urine metabolomics insight into acute kidney injury point to oxidative stress disruptions in energy generation and H 2 S availability  
   
نویسنده Gonzalez-Calero Laura ,Ramos-Barron Angeles ,Sanchez-Niño Maria D. ,Gomez-Alamillo Carlos ,García-Segura Juan Manuel ,Ortiz Alberto ,Arias Manuel ,Vivanco Fernando ,Alvarez-Llamas Gloria ,Martin-Lorenzo Marta
منبع journal of molecular medicine - 2017 - دوره : 95 - شماره : 12 - صفحه:1399 -1409
چکیده    Acute kidney injury (aki) is one of the main complications in acute care medicine and a risk factor for chronic kidney disease (ckd). aki incidence has increased; however, its diagnosis has limitations and physiopathological mechanisms are underexplored. we investigated urine samples, aiming to identify major metabolite changes during human aki evolution. metabolic signatures found were further explored for a potential link to severity of injury. twenty-four control subjects and 38 hospitalized patients with aki were recruited and urine samples were collected at the time of diagnosis, during follow-up and at discharge. nuclear magnetic resonance (nmr) was used in a first discovery phase for identifying potential metabolic differences. target metabolites of interest were confirmed by liquid chromatography-mass spectrometry (lc-ms/ms) in an independent group. underlying metabolic defects were further explored by kidney transcriptomics of murine toxic aki. urinary 2-hydroxybutyric acid, pantothenic acid, and hippuric acid were significantly downregulated and urinary n-acetylneuraminic acid, phosphoethanolamine, and serine were upregulated during aki. hippuric acid, phosphoethanolamine, and serine showed further downregulation/upregulation depending on the metabolite in acute tubular necrosis (atn) aki compared to prerenal aki. kidney transcriptomics disclosed decreased expression of cystathionase, cystathionine-β-synthase, and ethanolamine-phosphate cytidylyltransferase, and increased n-acetylneuraminate synthase as the potentially underlying cause of changes in urinary metabolites. a urinary metabolite panel identified aki patients and provided insight into intrarenal events. a urine fingerprint made up of six metabolites be related to pathophysiological changes in oxidative stress, energy generation, and h2s availability associated with aki.
کلیدواژه Metabolomics ,Urine ,Acute kidney injury ,Oxidative stress ,H2S
آدرس Department of Immunology, Spain, Universidad de Cantabria, Nephrology Department, Spain, Department of Nephrology/IRSIN, Spain, Universidad de Cantabria, Nephrology Department, Spain, Universidad Complutense, Department of Biochemistry and Molecular Biology I, Spain, Department of Nephrology/IRSIN, Spain, Universidad de Cantabria, Nephrology Department, Spain, Department of Immunology, Spain. Universidad Complutense, Department of Biochemistry and Molecular Biology I, Spain, Department of Immunology, Spain, Department of Immunology, Spain
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved