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ampk regulates anaphase central spindle length by phosphorylation of kif4a
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نویسنده
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li q.r. ,yan x.m. ,guo l. ,li j. ,zang y.
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منبع
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journal of molecular cell biology - 2018 - دوره : 10 - شماره : 1 - صفحه:2 -17
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چکیده
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Amp-activated protein kinase (ampk) is an energy sensor that couples the cellular energy state with basic biological processes. ampk is thought to be linked with cell division although the underlying mechanisms remain largely unknown. here, we show that ampk functionally participates throughout cell division and that ampk catalytic subunits, especially α2, are sequentially associated with separate mitotic apparatus. using quantitative phosphoproteomics analysis, we found that the strong direct substrate kif4a is phosphorylated by ampk at ser801. further analysis revealed that ampk and aurora b competitively phosphore-gulates kif4a during mitotic phase due to overlapping recognition motifs, resulting in the elaborate phosphoregulation for kif4a-dependent central spindle length control. given the intrinsic energy-sensing function of ampk, our study links the kif4a-dependent control of central spindle length with cellular glucose stress.
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کلیدواژه
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ampk ,central spindle length ,kif4a ,mitosis ,phosphoregulation
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آدرس
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wuhan university, college of life sciences, state key laboratory of virology, china. chinese academy of sciences, shanghai institute of materia medica, national center for drug screening, state key laboratory of drug research, china, chinese academy of sciences, institute of biochemistry and cell biology, shanghai institutes for biological sciences, cas center for excellence in molecular cell science, state key laboratory of cell biology, china, wuhan university, college of life sciences, state key laboratory of virology, china, chinese academy of sciences, shanghai institute of materia medica, national center for drug screening, state key laboratory of drug research, china, chinese academy of sciences, shanghai institute of materia medica, national center for drug screening, state key laboratory of drug research, china
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Authors
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