aniline-based inhibitors of influenza h1n1 virus acting on hemagglutinin-mediated fusion

Two series of easily accessible anilines were identified as inhibitors of influenza a virus subtype h1n1, and extensive chemical synthesis and analysis of the structure-activity relationship were performed. the compounds were shown to interfere with low ph-induced membrane fusion mediated by the h1 and h5 (group 1) hemagglutinin (ha) subtypes. a combination of virus resistance, ha interaction, and molecular dynamics simulation studies elucidated the binding site of these aniline-based influenza fusion inhibitors, which significantly overlaps with the pocket occupied by some h3 ha-specific inhibitors, indicating the high relevance of this cavity for drug design.