Intestinal lamina propria TcRγδ+ lymphocytes selectively express IL-10 and IL-17

Background: the characteristics and roles of gut lymphocytes have been only partly elucidated,in particular with regard to activation patterns. objectives: to characterize lymphocytes from various parts of the gut and examine their activation pattern as a network. methods: lymphocytes were isolated from the epithelium,the lamina propria,peyer's patches,mesenteric lymph nodes,the spleen,and peripheral blood of naïve mice. they were then characterized for t cell phenotype,t cell receptors (tcrs),activation markers,and cytokine production. results: the results showed a gradient of cells with an increasing proportion of tcrγδ+,cd8αα+ cells towards the gut lumen,with the highest number found in intraepithelial lymphocytes. these cells,together with lamina propria lymphocytes (lpls) were also characterized by a memory-like phenotype (cd25-cd45rblow and cd44high) and cd69 expression. cd8+ tcrγδ+ lpls produced il-10 and il-17,while tcrαß+ lpls were foxp3 positive. conclusions: gut lymphocytes express various receptors and cytokines according to their location. these specific features suggest a differential function for gut lymphocytes depending on their location. © 2010 esmon publicidad.