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Peptidoglycan from Staphylococcus aureus induces T H2 immune response in mice
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نویسنده
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matsui k. ,nishikawa a.
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منبع
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journal of investigational allergology and clinical immunology - 2012 - دوره : 22 - شماره : 2 - صفحه:80 -86
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چکیده
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Background and objective: atopic dermatitis patients have an increased number of type 2 helper (t h2) cells in their peripheral blood and superfi cial staphylococcus aureus colonization. the purpose of this study was to clarify the effects of peptidoglycan (peg) from s aureus on the induction of the t h2immune response in mice. methods: mice were primed with peg- and ovalbumin (ova)-pulsed langerhans cells (lcs) and given a booster ova injection 2 days later via the hind footpad. five days later,the cytokine response in the draining popliteal lymph nodes was investigated by reverse transcriptionpolymerase chain reaction and enzyme-linked immunosorbent assay (elisa). il-12 production from cultured lcs was detected by elisa and western blot analysis. results: administration of peg- and ova-pulsed lcs into the hind footpads of the mice induced a t h2-prone immune response as represented by the enhanced interleukin (il) 4 expression in the lymph nodes. we further showed that higher levels of il-12 p40 production by pegstimulated lcs relative to il-12 p70 (p35/p40) production were associated with the induction of the th2 immune response. the lc-derived il-12 p40 protein induced by peg stimulation was detected mainly as monomeric and homodimeric il-12 p40 subunits; other heterodimers including the il-12 p40 subunit,such as il-23,were undetected. conclusion: these results suggest that peg may have the ability to induce the development of th2 cells through insuffi cient production of il-12 p70 and excessive production by lcs of homodimeric il-12 p40,a known antagonist of bioactive il-12 p70,offering a possible explanation for the role of s aureus colonization in patients with atopic dermatitis. © 2012 esmon publicidad.
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کلیدواژه
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Il-12; Langerhans cells; Peptidoglycan; Staphylococcus aureus; T H2
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آدرس
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department of immunobiology,meiji pharmaceutical university,tokyo, Japan, department of immunobiology,meiji pharmaceutical university,tokyo, Japan
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Authors
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