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A prospective study in children with a severe form of atopic dermatitis: Clinical outcome in relation to cytokine gene polymorphisms
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نویسنده
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kayserova j. ,sismova k. ,zentsova-jaresova i. ,katina s. ,vernerova e. ,polouckova a. ,capkova s. ,malinova v. ,striz i. ,sediva a.
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منبع
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journal of investigational allergology and clinical immunology - 2012 - دوره : 22 - شماره : 2 - صفحه:92 -101
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چکیده
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Background and objective: the course of atopic dermatitis (ad) in childhood is characterized by typical changes in phenotype,including a shift from skin involvement to respiratory allergy usually around the third year of age. we thus designed a prospective study to monitor the outcome of severe ad and to investigate the association between cytokine gene polymorphisms and clinical manifestations. methods: clinical and laboratory follow-up of 94 patients with severe ad and 103 healthy controls was performed using routine methodology. allele,genotype,and haplotype frequencies of single nucleotide polymorphisms of 13 selected cytokine/receptor genes were analyzed using pcr with sequence-specifi c primers. results: in our study,genotypes of 7 polymorphisms-il-4 -1098g/t and -590c/t,il-6 -174c/g and nt565a/g,and il-10 -1082a/g,-819c/t,and -592a/c were signifi cantly associated with atopic ad (p<.05). a signifi cant association was also found for tnf-α aa and il-4 gc haplotypes and ad. we confi rm the progressive clinical improvement of ad together with a decrease in the severity index scorad (scoring atopic dermatitis) during childhood (p<.05). we found signifi cant differences between il-4rα +1902 a/g and positivity of tree pollen-specifi c ige (p<.05) in the ad group. moreover,a weak association was also found between il-10 -819c/t and il-10 -590a/c and the appearance of allergic rhinitis (p<0.1). conclusions: we confi rmed a clinical shift in allergic phenotype in the fi rst 3 years of life,and showed an association between il-4,il-6,and il-10 polymorphisms and ad. our data indicate that il-4α and il-10 polymorphisms may be considered predictive factors of respiratory allergy in children with ad. © 2012 esmon publicidad.
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کلیدواژه
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Allergic rhinitis; Allergy; Atopic dermatitis; Cytokines; Single nucleotide polymorphism
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آدرس
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department of immunology,charles university,2nd faculty of medicine,university hospital motol,prague, Czech Republic, department of immunology,charles university,2nd faculty of medicine,university hospital motol,prague, Czech Republic, department of immunology,charles university,2nd faculty of medicine,university hospital motol,prague, Czech Republic, department of applied mathematics and statistics,faculty of mathematics,physics and informatics,comenius university,bratislava,slovakia,institute of normal and pathological physiology,slovak academy of sciences,bratislava, Slovakia, department of immunology,charles university,2nd faculty of medicine,university hospital motol,prague, Czech Republic, department of immunology,charles university,2nd faculty of medicine,university hospital motol,prague, Czech Republic, paediatric department of dermatology,paediatric outpatient department,university hospital motol,prague, Czech Republic, department of paediatrics and adolescent medicine,1st faculty of medicine,general university hospital,prague, Czech Republic, department of immunogenetics,institute for clinical and experimental medicine,prague, Czech Republic, department of immunology,charles university,2nd faculty of medicine,university hospital motol,prague, Czech Republic
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Authors
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