Cellulose sulfate suppresses immunoglobulin E production by murine B lymphocytes in vitro

Background: immunoglobulin (ig) e plays an important role in the pathogenesis of allergic diseases such as atopic dermatitis and allergic asthma. we previously reported that a sulfate polysaccharide,fucoidan,suppressed ige production by murine b cells in vitro. however,the mechanism by which fucoidan suppresses ige production remains unclear. objective: we incorporated sulfate groups into cellulose and studied their biological characteristics in vitro to explore the possibility of converting biologically neutral polysaccharides to active reagents with antiallergic functions. material and methods: cellulose was chemically processed using n,n-dimethylformamide (dmf) and dmf-sulfurtrioxide and recovered as cellulose sulfate with a molecular weight of around 10 kda. we then studied the effect of cellulose sulfate on ige production from b cells,ige class-switching,and populations of ige-secreting b cells prepared from murine spleen. we also investigated the effects of sulfated cellulose on the production of interleukin (il) 4 and interferon (ifn) γ and the expression of t-bet mrna by splenic t cells. the cytotoxicity of cellulose sulfate was also examined. results: cellulose sulfate suppressed ige production in b cells stimulated with il-4 and anti-cd40 antibody by inhibiting ige class-switch recombination and decreasing the number of ige-secreting b cells in vitro. moreover,both cellulose sulfate and fucoidan suppressed il-4 production and enhanced ifn-γ production by murine t cells stimulated with anti-cd3 and anti-cd28 antibodies,despite the decrease in t-bet mrna expression. conclusions: cellulose gains an antiallergic effect on b cells and t cells with the addition of sulfate groups. © 2012 esmon publicidad.