Immune responses to Tyrophagus putrescentiae-induced airway inflammation in mice

Background: storage mites are a source of aeroallergens that affect patients with allergic rhinitis and asthma. tyrophagus putrescentiae is a causative factor of airway hypersensitivity,but the mechanisms and pathogenesis of t putrescentiae-induced allergy are not well understood. objective: this study aimed to develop a murine model of t putrescentiae-induced allergic asthma. methods: immune responses and physiologic variations in immunoglobulins (ig),leukocyte subpopulations,cytokines,gene expression,pulmonary function,and lung pathology were evaluated after intraperitoneal sensitization and intratracheal challenge with crude extract of t putrescentiae. results: after sensitization with aluminum hydroxide and challenge with t putrescentiae in mice,levels of t putrescentiae-specific ige and igg1 in sera increased significantly compared to the normal saline group (p<.01). values for inflammatory leukocytes (neutrophils and eosinophils) and cytokines (interleukin [il] 4,il-5,and il-13) increased significantly after sensitization. in terms of pulmonary function,pause values were significantly enhanced in t putrescentiae-sensitized mice after intratracheal challenge with t putrescentiae (p<.05). expression of type 2 helper t cell (th2)-related genes (il4,il5,il13,and rantes),th2-specific transcription factor (gata-3),and proinflammatory genes (il6),and th17-related genes (il17f) increased significantly after airway challenge. sensitization with t putrescentiae crude extract led to inflammation of lung tissue,thickening of the tracheal wall,and tracheal rupture. conclusions: intraperitoneal sensitization followed by intratracheal challenge with crude extract of t putrescentiae can induce airway inflammation in balb/c mice. the symptoms observed in a mouse model of allergic asthma,in terms of immune and clinical parameters,are reminiscent of the symptoms of allergic asthma in humans. a mouse model can be used to evaluate the therapeutic effectiveness of drugs on t putrescentiae-induced airway inflammation in humans. © 2013 esmon publicidad.