In vivo and in vitro testing with rAni s 1 can facilitate diagnosis of Anisakis simplex allergy

Background: traditional diagnostic tests such as skin prick tests (spt) and specific ige (sige) against whole anisakis simplex extract have low specificity. consequently,allergy to a simplex is overdiagnosed.objective: our aim was to compare tests used in component-resolved diagnosis.methods: we evaluated 34 patients with allergy to a simplex,15 patients with acute urticaria who were sensitized to a simplex but had no clinical history of allergy to a simplex,and 10 patients allergic to seafood. spt,sige (elisa and isac-112),and the basophil activation test (bat) were performed with a simplex whole extract and the molecular components rani s 1,rani s 3,and npen m 1. sensitivity and specificity were calculated and compared with different cutoffs.results: with the a simplex whole extract,spt,sige,and bat yielded specificity values of 72%,68%,and 70%,respectively,with a cutoff (wheal size) of 11.2 mm,an sige value of 7.9 kua/l,and a stimulation index of 1.9. specificity increased to 100% using the molecular component rani s 1 with spt,sige by elisa,and isac-112. neither rani s 3 sensitization nor cross-reactivity with pen m 1 was observed in patients sensitized to a simplex.conclusion: rani s 1 is recognized by 100% of our patients and is able to distinguish between patients allergic to a simplex and patients with acute urticaria who are sensitized to a simplex but have no clinical history of allergy to this parasite. © 2014 esmon publicidad.