Component-resolved diagnostic study of Dermatophagoides Pteronyssinus major allergen molecules in a southern Chinese cohort

Background and objective: little is known about component-resolved diagnosis (crd) for dermatophagoides pteronyssinus (der p) sensitization in the chinese population. we aimed to evaluate sensitization to der p components in southern china. methods: two-hundred immunotherapy-naïve patients with asthma and/or rhinitis positive to specific ige (sige) against der p extract,along with 20 der p-negative nonallergic healthy controls,were tested for sige against der p 1,der p 2,and der p 10 using immunocap 100. seventy-five were further examined with the immunocap immuno solid-phase allergen chip (isac). der p 10-positive patients were also tested for sige against crude extracts of cockroach,moth,and shrimp. results: in total,183 (91.5%) of the 200 patients were sensitized to der p 1 and/or der p 2. the proportion of positive results and the median level of sige against der p 1 were higher in children than in adults. der p 1 and der p 2 correlated with der p in sige levels. immunocap isac demonstrated 100% specificity and 84% sensitivity in detecting der p 1,der p 2,and der p10 compared with immunocap 100. sensitization to der p 10 correlated well with sige to shrimp,moths,cockroaches,pen m 1,bla g 7,and ani s 3. conclusions: the detection of der p 1 and der p 2 provided a good reflection of atopy to der p in a chinese cohort. sensitization to der p 10 may result from cross-reactivity with seafood and cockroaches in coastal southern china. immunocap isac may be a useful tool for crd,with comparable performance to immunocap 100. © 2015 esmon publicidad.