Relevance of allergenic sensitization to Cynodon dactylon and phragmites communis: Cross-reactivity with pooideae grasses

Background and objectives: the homologous group of sweet grasses belongs to the pooideae subfamily,but grass pollen species from other subfamilies can also cause allergy,such as cynodon dactylon (chloridoideae) and phragmites communis (arundinoideae). c dactylon and p communis have not been included in the sweet grasses homologous group because of their low cross-reactivity with other grasses. the aims of this study were to investigate the profile of sensitization to c dactylon and p communis in patients sensitized to grasses and to analyze cross-reactivity between these 2 species and temperate grasses. methods: patients were skin prick tested with a grass mixture (gm). specific ige to gm,c dactylon,p communis,cyn d 1,and phl p 1 was measured by immunocap. a pool of sera was used for the immunoblot assays. cross-reactivity was studied by elisa and immunoblot inhibition. results: thirty patients had sige to gm. twenty-four (80%) had positive results for c dactylon,27 (90%) for p communis,22 (73.3%) for ncyn d 1,and 92.9% for rphl p 1. bands were detected in the 3 extracts by immunoblot. inhibition of gm was not observed with c dactylon or p communis by immunoblot or elisa inhibition. when c dactylon or p communis were used in the solid phase,gm produced almost complete inhibition. conclusions: eighty percent of patients sensitized to grasses were also sensitized to c dactylon and 90% were sensitized to p communis. sensitization to these species seems to be induced by allergens different to those in sweet grasses. © 2016 esmon publicidad.