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Is microarray analysis really useful and sufficient to diagnose nut allergy in the Mediterranean area?
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نویسنده
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goikoetxea m.j. ,d’amelio c.m. ,martínez-aranguren r. ,gamboa p. ,garcia b.e. ,gómez f. ,fernández j. ,bartra j. ,parra a. ,alvarado m.i. ,alonso m.i. ,gonzález e. ,terrados s. ,moya c. ,blanca n. ,feo-brito f. ,villalba m. ,díaz-perales a. ,sanz m.l.
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منبع
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journal of investigational allergology and clinical immunology - 2016 - دوره : 26 - شماره : 1 - صفحه:31 -39
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چکیده
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Background: component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy. objective: to assess the diagnostic performance of a commercial protein microarray in the determination of specific ige (sige) in peanut,hazelnut,and walnut allergy. methods: sige was measured in 36 peanut-allergic,36 hazelnut-allergic,and 44 walnut-allergic patients by isac 112,and subsequently,sige against available components was determined by immunocap in patients with negative isac results. immunocap was also used to measure sige to ara h 9,cor a 8,and jug r 3 in a subgroup of lipid transfer protein (ltp)-sensitized nut-allergic patients (positive skin prick test to ltp-enriched extract). sige levels by immunocap were compared with isac ranges. results: most peanut-,hazelnut-,and walnut-allergic patients were sensitized to the corresponding nut ltp (ara h 9,66.7%; cor a 8,80.5%; jug r 3,84% respectively). however,isac did not detect sige in 33.3% of peanut-allergic patients,13.9% of hazelnut-allergic patients,or 13.6% of walnut-allergic patients. sige determination by immunocap detected sensitization to ara h 9,cor a 8,and jug r 3 in,respectively,61.5% of peanut-allergic patients,60% of hazelnut-allergic patients,and 88.3% of walnut-allergic patients with negative isac results. in the subgroup of peach ltp-sensitized patients,ara h 9 sige was detected in more cases by immunocap than by isac (94.4% vs 72.2%,p<.05). similar rates of cor a 8 and jug r 3 sensitization were detected by both techniques. conclusions: the diagnostic performance of isac was adequate for hazelnut and walnut allergy but not for peanut allergy. sige sensitivity against ara h 9 in isac needs to be improved. © 2016 esmon publicidad.
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کلیدواژه
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Hazelnut; Lipid transfer protein; Microarray; Peanut; Walnut
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آدرس
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department of allergy and clinical immunology,clinica universidad de navarra,instituto de investigación sanitaria de navarra,pamplona, Spain, department of allergy and clinical immunology,clinica universidad de navarra,instituto de investigación sanitaria de navarra,pamplona, Spain, department of allergy and clinical immunology,clinica universidad de navarra,instituto de investigación sanitaria de navarra,pamplona, Spain, allergy department,hospital de basurto,bilbao, Spain, allergy department,complejo hospitalario de navarra,instituto de investigación sanitaria de navarra,pamplona, Spain, allergy department,hospital regional universitario de málaga,ibima,málaga, Spain, allergy department,hospital general universitario de alicante,alicante, Spain, allergy,respiratory,and respiratory allergy department,hospital clinic,universitat de barcelona,institut d’investigacions biomédiques august pi i sunyer (idibaps),centro de investigaciones biomédicas en red de enfermedades respiratorias (ciberes), Spain, allergy department,hospital de a coruña,a coruña, Spain, allergy department,hospital virgen del puerto,plasencia,cáceres, Spain, allergy department,hospital de alcorcón,madrid, Spain, allergy department,hospital de fuenlabrada,madrid, Spain, allergy department,hospital ramón y cajal,madrid, Spain, allergy department,complejo hopitalario torrecárdenas,almeria, Spain, allergy department,hospital infanta leonor,madrid, Spain, allergy department,hospital general universitario de ciudad real,ciudad real, Spain, molecular biology department,universidad complutense,madrid, Spain, biotechnology department,centro de biotecnología y genómica de plantas,pozuelo de alarcón,madrid, Spain, department of allergy and clinical immunology,clinica universidad de navarra,instituto de investigación sanitaria de navarra,pamplona, Spain
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Authors
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