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Intra-coronary administration of tacrolimus markedly attenuates infarct size and preserves heart function in porcine myocardial infarction
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نویسنده
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chua s. ,leu s. ,sheu j.-j. ,lin y.-c. ,chang l.-t. ,kao y.-h. ,yen c.-h. ,tsai t.-h. ,chen y.-l. ,chang h.-w. ,sun c.-k. ,yip h.-k.
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منبع
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journal of inflammation - 2012 - دوره : 9 - شماره : 0
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چکیده
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Background: we test the hypothesis that intra-coronary tacrolimus administration can limit infarct size and preserve left ventricular ejection fraction (lvef) after acute myocardial infarction (ami) through ligating left anterior descending coronary artery (lad) in mini-pigs. methods: twelve male mini-pigs were randomized into ami-saline (mi-only) group and ami-tacrolimus (mi-tac) group that received intra-coronary saline (3.0 ml) and tacrolimus (0.5 mg in 2.5 ml saline) injection,respectively,beyond site of ligation 30 minutes after lad occlusion. results: larger infarct area was noted in mi-only group (p<0.001). inflammatory biomarkers at protein [oxidative stress,tumor necrotic factor-?,nuclear factor-?b],gene (matrix metalloproteinase-9,plasminogen activator inhibitor-1),and cellular (cd40+,cd68+ inflammatory cells) levels were remarkably higher in mi-only animals (p<0.01). conversely,anti-inflammatory biomarkers at gene level (interleukin-10),gene and protein level (endothelial nitric oxide synthase),and anti-oxidant biomarkers at both gene and protein levels [heme oxygenase 1,nad(p)h:quinone oxidoreductase] were lower in mi-only group (p<0.01). number of apoptotic nuclei and apoptotic biomarkers expressions at gene and protein levels (bax,caspase 3) were notably higher,whereas anti-apoptotic biomarkers at gene and protein levels (bcl-2),lvef,and fractional shortening were markedly lower in mi-only group (p<0.001). conclusion: intra-coronary administration of tacrolimus significantly attenuated infarct size and preserved lv function. © 2012 chua et al.; licensee biomed central ltd.
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آدرس
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department of internal medicine,kaohsiung chang gung memorial hospital,chang gung university college of medicine,kaohsiung, Taiwan, center for translational research in biomedical sciences,kaohsiung chang gung memorial hospital,chang gung university college of medicine,kaohsiung, Taiwan, department of surgery,kaohsiung chang gung memorial hospital,chang gung university college of medicine,kaohsiung, Taiwan, department of medical research,e-da hospital,i-shou university,kaohsiung, Taiwan, basic science,nursing department,meiho university,pingtung, Taiwan, department of medical research,e-da hospital,i-shou university,kaohsiung, Taiwan, department of biological science and technology,national pingtung university of science and technology,pingtung, Taiwan, department of internal medicine,kaohsiung chang gung memorial hospital,chang gung university college of medicine,kaohsiung, Taiwan, department of internal medicine,kaohsiung chang gung memorial hospital,chang gung university college of medicine,kaohsiung, Taiwan, department of biological sciences,national sun yat-sen university,kaohsiung, Taiwan, department of emergency medicine,e-da hospital,i-shou university,kaohsiung, Taiwan, department of internal medicine,kaohsiung chang gung memorial hospital,chang gung university college of medicine,kaohsiung,taiwan,center for translational research in biomedical sciences,kaohsiung chang gung memorial hospital,chang gung university college of medicine,kaohsiung, Taiwan
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Authors
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