Antioxidant and anti-inflammatory effects of selenium in oral buccal mucosa and small intestinal mucosa during intestinal ischemia-reperfusion injury

Background: the aim of this study were to investigate whether selenium treatment attenuates lipid peroxidation and downregulates the nf-κb pathway in small intestinal mucosa and to examine whether the effect of selenium is also observed in oral buccal mucosa,during small intestinal ir injury. materials and methods: eighteen rats were assigned into three groups: sham,ir,and ir + selenium. saline or selenium was administered through a tail vein. 24 hours later,the superior mesenteric artery was exposed and clamped in the ir and ir + selenium groups. after ischemic and reperfusion period,animals were sacrificed and oral buccal mucosa and small intestinal mucosa were harvested. results: glutathione peroxidase activity and cytoplasmic iκb-α expression was higher in the ir + selenium group than that in the ir group. a malondialdehyde level,cytoplasmic phosphorylated inhibitor κb-α,nuclear nf-κb p65 expressions,and nf-κb p65 dna-binding activity were lower in the ir + selenium group than those in the ir group. conclusion: a selenium treatment may cause increased gpx activity,attenuated lipid peroxidation,and downregulated the nf-κb pathway during small intestinal ir injury. furthermore,these therapeutic benefits of selenium can be observed in oral buccal mucosa as well as small intestinal mucosa. © 2014 kim et al.