In Vitro Antimicrobial Activity of Iris pseudacorus and Urtica dioica

Background: in this study, the effects of antibacterial activity of urtica dioica and iris pseudacorus essential oils, native plant northern of iran, were investigated for some selected bacteria. material and methods: the influence of essential oils was tested by the using of disk diffusion and micro-broth dilution methods against standard strains of the picked out bacteria. gas chromatography/mass spectroscopy (gc/ms) analysis, bioactivity determination, minimum inhibitory concentrations (mic) and minimum bactericidal concentration (mbc) of essential oils were utilized for this goal. results: this study showed that, inhibition zone diameter varied from 11 to 19 mm and 9 to 17 mm for urtica dioica and iris pseudacorus respectively. in contrast, this figure fluctuated from 19 to 28 mm and 7 to 17 mm for gentamicin and ampicillin separately. by the application of micro-broth dilution technique, mics for 1% essential oils were 1.8-7.5 ?g/ml and 3.75-15 ?g/ml for, urtica dioica and iris pseudacorus against gram-positive and gram-negative bacteria individually. furthermore, the mbcs of herbal essences were 1.8-15 ?g/ml for, urtica dioica and 15-30 ?g/ml for iris. conclusion: the application of essential oils for the bio-control of diseases, as a novel emerging alternative to antimicrobial treatments, lead to safer and more environmental management for infective diseases.