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Synergistic Effect of Sodium Butyrate and Thalidomide in the Induction of Fetal Hemoglobin Expression in Erythroid Progenitors Derived from Cord Blood CD133 + Cells
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نویسنده
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Dehghanifard Ali ,Kaviani Saeid ,Noruzinia Mehrdad ,Soleimani Masoud ,Abroun Saeid ,Hajifathali Abbas ,Pourfathollah Ali Akbar ,Mortazavi Yousef ,Ahmadvand Mohammad ,Mahmoodinia-Meymand Maryam ,Kaviani Zeinab ,Farshdousti-Hagh Majid ,Saki Najmaldin
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منبع
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zahedan journal of research in medical sciences - 1391 - دوره : 14 - شماره : 7 - صفحه:29 -33
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چکیده
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Background: the use of drugs with the ability to induce production of fetal hemoglobin as a novel therapeutic approach in treating b-hemoglobinopathies is considered. ?-globin gene expression inducer drugs including sodium butyrate and thalidomide can reduce additional ?-globin chains accumulation in erythroid precursors. materials and methods: in this experimental study, macs kit was used to isolate cd133+ cells of umbilical cord blood. further, the effect of two drugs of thalidomide and sodium butyrate were separately and combined studied on the induction of quantitative expression of b-globin and ?-globin genes in erythroid precursor cells derived from cd133+ stem cells in-vitro. for this purpose, the technique sybr green real-time pcr was used.results: flow cytometry results showed that approximately 95% of purified cells were cd133+. real-time pcr results also showed the increased levels of ?-globin mrna in the cell groups treated with thalidomide, sodium butyrate and combination of drugs as 2.6 and 1.2 and 3.5 times respectively, and for b-globin gene, it is respectively 1.4 and 1.3 and 1.6 times compared with the control group (p < 0.05).conclusion: the study results showed that the mentioned drug combination can act as a pharmaceutical composition affecting the induction of fetal hemoglobin expression in erythroid precursor cells derived from cd133 + cells.
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کلیدواژه
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CD133+ cells ,?-globin gene ,Sodium butyrate ,Thalidomide
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آدرس
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tarbiat modares university, Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran, ایران, tarbiat modares university, Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran, ایران, tarbiat modares university, Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran, ایران, tarbiat modares university, Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran, ایران, tarbiat modares university, Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran, ایران, shahid beheshti university, Department of Hematology and Oncology, School of Medical Sciences, Shahid Beheshti University, Tehran, Iran, ایران, tarbiat modares university, Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran, ایران, zanjan university of medical sciences, Department of Pathology, Zanjan University of Medical Sciences, Zanjan, Iran, ایران, zahedan university of medical sciences, Department of Laboratory Sciences, Zahedan University of Medical Sciences, Zahedan, Iran, ایران, Department of Biology, Stem cell Research Center, , Department of Biology, Stem cell Research Center, Sarem Hospital, Tehran, Iran, ایران, alborz university of medical sciences, Department of Internal Medicine, Alborz University of Medical Sciences, Karaj, Iran, ایران, tabriz university of medical sciences, Laboratory of Hematology and Blood Banking, Tabriz University of Medical Sciences, Tabriz, Iran, ایران, ahvaz jundishapur university of medical sciences, Thalassemia and Hemoglobinopathy Research Center, Jundi Shapur University of Medical Sciences, Ahvaz, Iran, ایران
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Authors
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