effects of vitamins e and c on freezed-thawed immature mice testis

Background: the freezing of immature testis tissue and then the transplant of it can be considered as a major step in fertility preservation for young boys with cancer. objectives: the objective of this study was to investigate antioxidant effects of vitamins e and c on testis tissue structure in the two types of freezing-thawing (fast and slow). materials and methods: in this experimental study, 16 immature male mice (6 to 8 day-old) surgery, and testes were removed. the testes were divided randomly into 4 groups: control group and three experimental groups including: vitamin e, vitamins c and vitamins e and c. testes placed in 4 prepared freezing solutions. these solutions, which were: 0.1 mm solution of vitamin e, 1 mm solution of vitamin c, solution of the vitamins e and c with mentioned doses and control solution, were prepared in the dmem (dulbecco’s modified eagle medium) base culture. of each group were 4 testes into fast freezing-thawing procedure and 4 testes were into slow freezing-thawing procedure. finally these testes, for analyzing injury, were stained with hematoxylin-eosin. results: in the fast freezing-thawing procedure, seminiferous tubules injury significantly reduced in any of the three experimental groups compared to the control group (p = 0.01). at the slow freezing-thawing procedure, seminiferous tubules injury any of the three experimental groups significantly reduced compared to the control group (p = 0.01). in both procedures, there was no significant difference between the three experimental groups. conclusions: it seems that vitamins e and c due to its antioxidant properties protects seminiferous tubules from oxidative injury.