Comparison of the multi-drug resistant human hepatocellular carcinoma cell line Bel-7402/ADM model established by three methods

Background. to compare the biological characteristics of three types of human hepatocellular carcinoma multi-drug resistant cell sub-lines bel-7402/adm models established by three methods. methods. established human hepatocellular carcinoma adriamycin (adm) multi-drug resistant cell sub-lines models bel-7402/admv,bel-7402/admland bel-7402/admsby three methods of in vitro concentration gradient increased induction,nude mice liver-implanted induction and subcutaneous-implanted induction respectively. phase contrast microscopy was used to observe the cells and the mtt (methyl thiazolyl tetrazolium) method was used to detect drug resistance of the three different sub-lines of cells. results. the three groups of drug resistant cells,bel-7402/admv,bel-7402/adml and bel-7402/adms generated cross-resistance to adm and cddp (cis-diaminedichloroplatinum),but showed a significant difference in resistance to bel-7402 ic50 value (p < 0.01). the doubling times were significantly extended compared to the parent cell line (39 h) and were 65 h (bel-7402/admv),46 h (bel-7402/adml),and 45 h (bel-7402/adms). the excretion rates of adm were significantly increased compared with the parent cell (34.14%) line and were 81.06% (bel-7402/admv),66.56% (bel-7402/adm l) and 61.56% (bel-7402/adms). expression of p-gp and mrp in the three groups of resistant cells was significantly enhanced (p < 0.01). there was no significant variation in the expression of gsh/gst (p > 0.05). conclusions. stable resistance was involved in the resistant cell line model established by the above three methods. liver implantation was a good simulation of human hepatocellular and proved to be an ideal model with characteristics similar to human hepatocellular biology and the pharmacokinetics of anticancer drugs. © 2010 zhong et al; licensee biomed central ltd.