The HPB-AML-I cell line possesses the properties of mesenchymal stem cells

Background. in spite of its establishment from the peripheral blood of a case with acute myeloid leukemia (aml)-m1,hpb-aml-i shows plastic adherence with spindle-like morphology. in addition,lipid droplets can be induced in hpb-aml-i cells by methylisobutylxanthine,hydrocortisone,and indomethacin. these findings suggest that hpb-aml-i is similar to mesenchymal stem cells (mscs) or mesenchymal stromal cells rather than to hematopoietic cells. methods. to examine this possibility,we characterized hpb-aml-i by performing cytochemical,cytogenetic,and phenotypic analyses,induction of differentiation toward mesenchymal lineage cells,and mixed lymphocyte culture analysis. results. hpb-aml-i proved to be negative for myeloperoxidase,while surface antigen analysis disclosed that it was positive for msc-related antigens,such as cd29,cd44,cd55,cd59,and cd73,but not for cd14,cd19,cd34,cd45,cd90,cd105,cd117,and hla-dr. karyotypic analysis showed the presence of complicated abnormalities,but no reciprocal translocations typically detected in aml cases. following the induction of differentiation toward adipocytes,chondrocytes,and osteocytes,hpb-aml-i cells showed,in conjunction with extracellular matrix formation,lipid accumulation,proteoglycan synthesis,and alkaline phosphatase expression. mixed lymphocyte culture demonstrated that cd3+ t-cell proliferation was suppressed in the presence of hpb-aml-i cells. conclusions. we conclude that hpb-aml-i cells appear to be unique neoplastic cells,which may be derived from mscs,but are not hematopoietic progenitor cells. © 2010 ardianto et al; licensee biomed central ltd.