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   The HPB-AML-I cell line possesses the properties of mesenchymal stem cells  
   
نویسنده ardianto b. ,sugimoto t. ,kawano s. ,kasagi s. ,jauharoh s.n. ,kurimoto c. ,tatsumi e. ,morikawa k. ,kumagai s. ,hayashi y.
منبع journal of experimental and clinical cancer research - 2010 - دوره : 29 - شماره : 1
چکیده    Background. in spite of its establishment from the peripheral blood of a case with acute myeloid leukemia (aml)-m1,hpb-aml-i shows plastic adherence with spindle-like morphology. in addition,lipid droplets can be induced in hpb-aml-i cells by methylisobutylxanthine,hydrocortisone,and indomethacin. these findings suggest that hpb-aml-i is similar to mesenchymal stem cells (mscs) or mesenchymal stromal cells rather than to hematopoietic cells. methods. to examine this possibility,we characterized hpb-aml-i by performing cytochemical,cytogenetic,and phenotypic analyses,induction of differentiation toward mesenchymal lineage cells,and mixed lymphocyte culture analysis. results. hpb-aml-i proved to be negative for myeloperoxidase,while surface antigen analysis disclosed that it was positive for msc-related antigens,such as cd29,cd44,cd55,cd59,and cd73,but not for cd14,cd19,cd34,cd45,cd90,cd105,cd117,and hla-dr. karyotypic analysis showed the presence of complicated abnormalities,but no reciprocal translocations typically detected in aml cases. following the induction of differentiation toward adipocytes,chondrocytes,and osteocytes,hpb-aml-i cells showed,in conjunction with extracellular matrix formation,lipid accumulation,proteoglycan synthesis,and alkaline phosphatase expression. mixed lymphocyte culture demonstrated that cd3+ t-cell proliferation was suppressed in the presence of hpb-aml-i cells. conclusions. we conclude that hpb-aml-i cells appear to be unique neoplastic cells,which may be derived from mscs,but are not hematopoietic progenitor cells. © 2010 ardianto et al; licensee biomed central ltd.
آدرس department of pathology,graduate school of medicine,kobe university,kobe,japan,department of clinical pathology and immunology,graduate school of medicine,kobe university,chuo-ku,kobe 650-0017, Japan, department of clinical pathology and immunology,graduate school of medicine,kobe university,chuo-ku,kobe 650-0017, Japan, department of clinical pathology and immunology,graduate school of medicine,kobe university,chuo-ku,kobe 650-0017, Japan, department of clinical pathology and immunology,graduate school of medicine,kobe university,chuo-ku,kobe 650-0017, Japan, department of clinical pathology and immunology,graduate school of medicine,kobe university,chuo-ku,kobe 650-0017, Japan, department of clinical pathology and immunology,graduate school of medicine,kobe university,chuo-ku,kobe 650-0017, Japan, division of clinical nutrition,department of nutrition,sagami women's university,sagamihara, Japan, division of clinical nutrition,department of nutrition,sagami women's university,sagamihara, Japan, department of clinical pathology and immunology,graduate school of medicine,kobe university,chuo-ku,kobe 650-0017, Japan, department of pathology,graduate school of medicine,kobe university,kobe, Japan
 
     
   
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