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Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice
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نویسنده
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zhou s. ,li s. ,liu z. ,tang y. ,wang z. ,gong j. ,liu c.
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منبع
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journal of experimental and clinical cancer research - 2010 - دوره : 29 - شماره : 1
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چکیده
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Objective. the purpose of the study was to explore the anti-tumor effect of ultrasound -targeted microbubble destruction mediated herpes simplex virus thymidine kinase (hsv-tk) suicide gene system on mice hepatoma. methods. forty mice were randomly divided into four groups after the models of subcutaneous transplantation tumors were estabilished: (1) pbs; (2) hsv-tk (3) hsv-tk+ ultrasound (hsv-tk+us); (4) hsv-tk+ultrasound+microbubbles (hsv-tk+us+mb). the tk protein expression in liver cancer was detected by western-blot. applying tunel staining detected tumor cell apoptosis. at last,the inhibition rates and survival time of the animals were compared among all groups. results. the tk protein expression of hsv-tk+mb+us group in tumor-bearing mice tissues were significantly higher than those in other groups. the tumor inhibitory effect of ultrasound-targeted microbubble destruction mediated hsv-tk on mice transplantable tumor was significantly higher than those in other groups (p < 0.05),and can significantly improve the survival time of tumor-bearing mice. conclusion. ultrasound-targeted microbubble destruction can effectively transfect hsv-tk gene into target tissues and play a significant inhibition effect on tumors,which provides a new strategy for gene therapy in liver cancer. © 2010 zhou et al; licensee biomed central ltd.
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آدرس
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department of hepatobiliary surgery,second affiliated hospital,chongqing medical university,76 linjiang road,yuzhong district,chongqing 400010, China, department of hepatobiliary surgery,second affiliated hospital,chongqing medical university,76 linjiang road,yuzhong district,chongqing 400010, China, department of hepatobiliary surgery,second affiliated hospital,chongqing medical university,76 linjiang road,yuzhong district,chongqing 400010, China, department of hepatobiliary surgery,second affiliated hospital,chongqing medical university,76 linjiang road,yuzhong district,chongqing 400010, China, institutional of ultrasound imaging,second affiliated hospital,chongqing medical university,76 linjiang road,yuzhong district,chongqing 400010, China, department of hepatobiliary surgery,second affiliated hospital,chongqing medical university,76 linjiang road,yuzhong district,chongqing 400010, China, department of hepatobiliary surgery,second affiliated hospital,chongqing medical university,76 linjiang road,yuzhong district,chongqing 400010, China
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Authors
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