Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells

Aims: here we aimed to firstly investigate the role of mir-27a in proliferation and multidrug resistance of gastric cancer cells. methods: the role of mir-27a in gastric cancer cells was detected using mtt assay,soft agar assay,flow cytometry assay,nude mice assay,real-time pcr,western blot and reporter gene assay,etc. results: down-regulation of mir-27a could inhibit porliferation of gastric cancer cells in vitro and in vivo. down-regulation of mir-27a could also confer sensitivity of drugs on gastric cancer cells,and might increase accumulation and decrease releasing amount of adriamycin in gastric cancer cells. down-regulation of mir-27a could significantly decrease the expression of p-glycoprotein and the transcriptional activity of cyclin d1,and up-regulate the expression of p21. conclusions: mir-27a might play important roles in porliferation and drug resistance of gastric cancer. mir-27a might be considered as a useful target for cancer therapy. © 2011 zhao et al; licensee biomed central ltd.