Multi-drug resistance 1 genetic polymorphism and prediction of chemotherapy response in Hodgkin's lymphoma

Background: the human multi-drug resistance gene (mdr1),which encodes the major trans-membrane transporter p-glycoprotein (p-gp),was found to be associated with susceptibility to cancer and response to chemotherapy. the c3435t polymorphism of mdr1 gene was correlated with expression levels and functions of p-gp. here,we studied the association between mdr1 c3435t polymorphism and susceptibility to hodgkin lymphoma (hl) and patient's response to abvd chemotherapy regimen. methods. a total of 130 paraffin embedded tissue samples collected from hl patients were analyzed to identify the c3435t polymorphism. as a control group,120 healthy subjects were enrolled in the study. the c3435t polymorphism was genotyped by polymerase chain reaction and restriction fragment length polymorphism (pcr-rflp) method. data analysis was carried out using the statistical package spss version 17 to compute all descriptive statistics. chi-square and fisher exact tests were used to evaluate the genotype distribution and allele frequencies of the studied polymorphism. results: these studies revealed that the frequency of t allele was significantly higher in hl patients compared to the controls (p < 0.05). in addition,the frequency of ct and tt genotypes were also significantly higher in hl patients compared to the controls (p < 0.05). no association between c3435t polymorphism and response to abvd was detected among hl patients (p > 0.05). conclusions: these results suggest that mdr1 c3435t polymorphism might play a role in hl occurrence; however this polymorphism is not correlated with the clinical response to abvd. © 2011 mhaidat et al; licensee biomed central ltd.