MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells

Background: this study was performed to investigate the effect of microrna-203 (mir-203) on cell proliferation and migration in triple-negative breast cancer (tnbc). methods: real-time pcr was performed to detect the expression of mir-203 in tnbc cell lines. mir-203 precursor and control microrna (mirna) were transfected into triple-negative breast cancer (tnbc) cell lines and the effects of mir-203 up-regulation on the proliferation and migration of cells were investigated. meanwhile,the mrna and protein levels of baculoviral iap repeat-containing protein 5 (birc5) and lim and sh3 domain protein 1 (lasp1) were measured. luciferase assays were also performed to validate birc5 and lasp1 as mir-203 targets. results: both mir-203 and birc5 sirna signicantly inhibited cell proliferation in tnbc cells. both mir-203 and lasp1 sirna signicantly inhibited cell migration in tnbc cells,also. moreover,up-regulated of birc5 and lasp1 was able to abrogate the effects induced by transfection with the mir-203 precursor. conclusions: these data suggest that mir-203 may function as a tumor suppressor in tnbc cells. thus,mir-203 could be a potential therapeutic target for this disease. © 2012 wang et al.; licensee biomed central ltd.