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MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells
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نویسنده
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wang c. ,zheng x. ,shen c. ,shi y.
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منبع
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journal of experimental and clinical cancer research - 2012 - دوره : 31 - شماره : 1
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چکیده
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Background: this study was performed to investigate the effect of microrna-203 (mir-203) on cell proliferation and migration in triple-negative breast cancer (tnbc). methods: real-time pcr was performed to detect the expression of mir-203 in tnbc cell lines. mir-203 precursor and control microrna (mirna) were transfected into triple-negative breast cancer (tnbc) cell lines and the effects of mir-203 up-regulation on the proliferation and migration of cells were investigated. meanwhile,the mrna and protein levels of baculoviral iap repeat-containing protein 5 (birc5) and lim and sh3 domain protein 1 (lasp1) were measured. luciferase assays were also performed to validate birc5 and lasp1 as mir-203 targets. results: both mir-203 and birc5 sirna signicantly inhibited cell proliferation in tnbc cells. both mir-203 and lasp1 sirna signicantly inhibited cell migration in tnbc cells,also. moreover,up-regulated of birc5 and lasp1 was able to abrogate the effects induced by transfection with the mir-203 precursor. conclusions: these data suggest that mir-203 may function as a tumor suppressor in tnbc cells. thus,mir-203 could be a potential therapeutic target for this disease. © 2012 wang et al.; licensee biomed central ltd.
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کلیدواژه
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baculoviral IAP repeat-containing protein 5; Lim and SH3 domain protein 1; Migration; MiR-203; Proliferation; Triple-negative breast cancer
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آدرس
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department of breast oncology,tianjin medical university,cancer hospital,huanhuxi ave,tianjin,300060, China, department of thyroid and neck tumor,tianjin medical university,cancer hospital,huanhuxi ave,tianjin,300060, China, department of medical oncology,chinese armed police medical institute affiliated hospital,tianjin,300100, China, tianjin cancer institute,huanhuxi ave,tianjin,300060, China
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Authors
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