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Alpha B-crystallin is a new prognostic marker for laryngeal squamous cell carcinoma
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نویسنده
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mao y. ,zhang d.-w. ,lin h. ,xiong l. ,liu y. ,li q.-d. ,ma j. ,cao q. ,chen r.-j. ,zhu j. ,feng z.-q.
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منبع
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journal of experimental and clinical cancer research - 2012 - دوره : 31 - شماره : 1
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چکیده
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Background: alpha b-crystallin (αb-crystallin) has been suggested to play an important role in the development of solid tumors. however,the association between αb-crystallin expression and clinicopathological characteristics of human laryngeal carcinoma is not well defined. this study aimed to examine the expression of αb-crystallin in human laryngeal squamous cell carcinoma (lscc) and investigate the relationship between its expression and the prognosis of lscc. methods. real-time polymerase chain reaction (six lscc samples,six tumor-adjacent normal samples) and immunohistochemistry by tissue microarrays (109 lscc samples and 28 tumor-adjacent normal samples) were performed to characterize expression of the αb-crystallin gene in lscc. kaplan-meier survival and cox regression analyses were carried out to evaluate the prognosis of lscc. results: real-time polymerase chain reaction and immunohistochemistry analysis showed that the expression of αb-crystallin in lscc was significantly higher than that in tumor-adjacent normal tissues. moreover,the expression level of αb-crystallin protein in lscc was significantly related to alcohol consumption (p = 0.022),tumor differentiation (p = 0.007),ptnm stage (p = 0.041) and 5 years' survival (p =0.030). cox multi-factor analysis showed that αb-crystallin (p = 0.013),as well as ptnm stage (p =0.027) and lymphatic metastasis (p = 0.015) were independent prognosis factors for lscc. conclusions: the data suggest that αb-crystallin expression is correlated with malignant phenotypes of lscc and it may serve as a novel prognostic factor for lscc. © 2012 mao et al.
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کلیدواژه
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Alpha B-crystallin gene; Immunohistochemistry; Laryngeal carcinoma; Prognosis; qPCR; RT-PCR
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آدرس
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department of otolaryngology-head and neck surgery,jiangsu province official hospital,no.65 jiangsu road,nanjing 210029, China, department of otolaryngology-head and neck surgery,second affiliated hospital,nanjing medical university,no.121 jiang jia yuan,nanjing 210011, China, jiangsu provincial blood center,no.179 longpan road,nanjing 210042, China, department of pathology,second affiliated hospital,nanjing medical university,no.121 jiang jia yuan,nanjing 210011, China, department of otolaryngology-head and neck surgery,jiangsu province official hospital,no.65 jiangsu road,nanjing 210029, China, department of otolaryngology-head and neck surgery,jiangsu province official hospital,no.65 jiangsu road,nanjing 210029, China, department of otolaryngology-head and neck surgery,yiji shan hospital,wannan medical college,no.92 zheshan xi road,wuhu 241000, China, department of otolaryngology-head and neck surgery,second affiliated hospital,nanjing medical university,no.121 jiang jia yuan,nanjing 210011, China, department of otolaryngology-head and neck surgery,second affiliated hospital,nanjing medical university,no.121 jiang jia yuan,nanjing 210011, China, key laboratory of cancer biomarkers,nanjing medical university,ministry of health,no.140 hanzhong road,nanjing 210029,china,huadong medical institute of biotechnology,no.293 zhongshan dong road,nanjing 210002, China, key laboratory of cancer biomarkers,nanjing medical university,ministry of health,no.140 hanzhong road,nanjing 210029, China
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