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Effects of ectopic HER-2/neu gene expression on the COX-2/PGE 2/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
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نویسنده
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li s. ,ma x. ,ma l. ,wang c. ,he y. ,yu z.
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منبع
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journal of experimental and clinical cancer research - 2013 - دوره : 32 - شماره : 1
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چکیده
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Abstract. objectives. to investigate the role of her-2/neu-mediated cox-2/p450arom signal in estrogen-dependent endometrial carcinoma. methods. the recombinant eukaryotic expression vector,pcdna3.1-her-2/neu,was constructed and transfect to ishikawa endometrial carcinoma cells. the expression of cox-2 and p450arom in transfected cells were detected by real-time pcr and western blotting. the levels of estrogen in cell supernatants were detected by elisa. results: over-expression of her-2/neu in transfected cells was confirmed by real-time pcr and western blotting. the levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of cox-2 and p450arom expression in transfected cells. conclusion: her-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the cox-2/p450arom signal. © 2013 li et al.; licensee biomed central ltd.
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کلیدواژه
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COX-2; Endometrial carcinoma cell; Estradiol; HER-2/neu; P450arom
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آدرس
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department of gynecology and obstetrics,shengjing hospital of china medical university,shenyang,110004, China, department of gynecology and obstetrics,shengjing hospital of china medical university,shenyang,110004, China, department of gynecology and obstetrics,shengjing hospital of china medical university,shenyang,110004, China, department of gynecology and obstetrics,shengjing hospital of china medical university,shenyang,110004, China, department of gynecology and obstetrics,shengjing hospital of china medical university,shenyang,110004, China, department of gynecology and obstetrics,shengjing hospital of china medical university,shenyang,110004, China
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Authors
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