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   Detection of EGFR mutations in circulating free DNA by PNA-mediated PCR clamping  
   
نویسنده kim h.-r. ,lee s.y. ,hyun d.-s. ,lee m.k. ,lee h.-k. ,choi c.-m. ,yang s.-h. ,kim y.-c. ,lee y.c. ,kim s.y. ,jang s.h. ,lee j.c. ,lee k.y.
منبع journal of experimental and clinical cancer research - 2013 - دوره : 32 - شماره : 1
چکیده    Background: epidermal growth factor receptor (egfr)-activating mutations are major determinants in predicting the tumor response to egfr tyrosine kinase inhibitors in non-small cell lung cancer (nsclc). noninvasive test for the detection of egfr mutations is required,especially in nsclc patients from whom tissue is not available. in this study,we assessed the feasibility of detection of egfr mutations in free dna circulating in plasma. methods. plasma samples of 60 patients with partial response to gefitinib were analyzed to detect egfr-activating mutations in exons 19 and 21. forty (66.7%) of patients had tumor egfr mutation results. egfr mutations in plasma were detected using the peptide nucleic acid (pna)-mediated polymerase chain reaction (pcr) clamping method. all clinical data and plasma samples were obtained from 11 centers of the korean molecular lung cancer group (kmlcg). results: of the 60 patients,39 were female and the median age was 62.5 years. forty-three patients never smoked,53 had adenocarcinomas,and seven had other histologic types. egfr-activating mutation was detected in plasma of 10 cases (exon 19 deletion in seven and exon 21 l858r point mutation in three). it could not be found in plasma after treatment for 2 months. when only patients with confirmed egfr mutation in tumor were analyzed,17% (6 of 35) of them showed positive plasma egfr mutation and the mutation type was completely matched with that in tumor. there was no statistically significant difference in clinical parameters between patients with egfr mutations in plasma and those without egfr mutations. conclusions: the detection rate of egfr mutations from plasma was not so high despite highly sensitive egfr mutation test suggesting that more advances in detection methods and further exploration of characteristics of circulating free dna are required. © 2013 kim et al.; licensee biomed central ltd.
کلیدواژه EGFR mutation; Non-small cell lung cancer; Plasma; PNA-mediated PCR clamping method
آدرس korea cancer center hospital,seoul, South Korea, korea university guro hospital,seoul, South Korea, daegu catholic university medical center,daegu, South Korea, pusan national university hospital,busan, South Korea, inje university busan paik hospital,busan, South Korea, department of oncology,college of medicine,university of ulsan,388-1 pungnap-2 dong,songpa-gu,seoul 138-736, South Korea, wonkwang university hospital,iksan, South Korea, chonnam national university,hwasun hospital,hwasun-gun, South Korea, chonbuk national university hospital,chonju, South Korea, chungnam national university hospital,daejeon, South Korea, hallym university sacred heart hospital,anyang, South Korea, department of oncology,college of medicine,university of ulsan,388-1 pungnap-2 dong,songpa-gu,seoul 138-736, South Korea, department of internal medicine,konkuk university medical center,4-12 hwayang-dong,gwangjin-gu,seoul 143-729, South Korea
 
     
   
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