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External Quality Assessment (EQA) program for the preanalytical and analytical immunohistochemical determination of HER2 in breast cancer: An experience on a regional scale
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نویسنده
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terrenato i. ,arena v. ,pizzamiglio s. ,pennacchia i. ,perracchio l. ,buglioni s. ,ercolani c. ,sperati f. ,costarelli l. ,bonanno e. ,baldini d. ,candia s. ,crescenzi a. ,dal mas a. ,di cristofano c. ,gomes v. ,grillo l.r. ,pasquini p. ,pericoli m.n. ,ramieri m.t. ,di stefano d. ,ruco l. ,scarpino s. ,vitolo d. ,d'amati g. ,paradiso a. ,verderio p. ,mottolese m.
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منبع
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journal of experimental and clinical cancer research - 2013 - دوره : 32 - شماره : 1
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چکیده
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Background: an external quality assessment (eqa) program was developed to investigate the state of the art of her2 immunohistochemical determination in breast cancer (bc) in 16 pathology departments in the lazio region (italy). this program was implemented through two specific steps to evaluate her2 staining (step 1) and interpretation (step 2) reproducibility among participants. methods. the management activities of this eqa program were assigned to the coordinating center (cc),the revising centers (rcs) and the participating centers (pcs). in step 1,4 bc sections,selected by rcs,were stained by each pc using their own procedures. in step 2,each pc interpreted her2 score in 10 bc sections stained by the cc. the concordance pattern was evaluated by using the kappa category-specific statistic and/or the weighted kappa statistic with the corresponding 95% jackknife confidence interval. results: in step 1,a substantial/almost perfect agreement was reached between the pcs for scores 0 and 3+ whereas a moderate and fair agreement was observed for scores 1+ and 2+,respectively.in step 2,a fully satisfactory agreement was observed for 6 out of the 16 pcs and a quite satisfactory agreement was obtained for the remaining 10 pcs. conclusions: our findings highlight that in the whole her2 evaluation process the two intermediate categories,scores 1+ and 2+,are less reproducible than scores 0 and 3+. these findings are relevant in clinical practice where the choice of treatment is based on her2 positivity,suggesting the need to share evaluation procedures within laboratories and implement educational programs. © 2013 terrenato et al.; licensee biomed central ltd.
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کلیدواژه
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Breast Neoplasm/drug therapy; Immunohistochemistry/ methods; Quality control; Receptor erbB-2/analysis; Reproducibility of results
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آدرس
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biostatistic unit,regina elena national cancer institute,via elio chianesi 53,rome, Italy, institute of pathology,catholic university of sacred heart,largo agostino gemelli 8,rome, Italy, unit of medical statistics,biometry and bioinformatics,fondazione irccs istituto nazionale dei tumori,milan, Italy, institute of pathology,catholic university of sacred heart,largo agostino gemelli 8,rome, Italy, pathology department,regina elena national cancer institute,via elio chianesi 53,rome,00144, Italy, pathology department,regina elena national cancer institute,via elio chianesi 53,rome,00144, Italy, pathology department,regina elena national cancer institute,via elio chianesi 53,rome,00144, Italy, biostatistic unit,regina elena national cancer institute,via elio chianesi 53,rome, Italy, department of pathology,san giovanni-addolorata hospital,via dell'amba aradam 9,rome, Italy, anatomic pathology,dept of biomedicine and prevention,university of rome tor vergata,viale oxford 81,rome, Italy, pathology department,san filippo neri hospital,via martinotti 20,rome, Italy, pathology department,sandro pertini hospital,via dei monti tiburtini 385,rome, Italy, pathology department,regina apostolorum hospital,via s.francesco 50,albano laziale,rome, Italy, pathology department,san salvatore hospital,via vetoio-coppito,l'aquila, Italy, department of experimental medicine,sapienza university of rome,i.c.o.t,via franco faggiana 68,latina, Italy, pathology department,bel colle hospital,strada s. martinese,viterbo, Italy, pathology department,san camillo forlanini hospital,via gianicolense 1,rome, Italy, pathology department,coelio military hospital,piazza cellimontana 50,rome, Italy, pathology department,s.maria goretti hospital,via guido reni 1,latina, Italy, pathology department,asl frosinone,viale mazzini 94,frosinone, Italy, department of cytology and histology,university of rome la sapienza,ospedale s. andrea,via di grottarossa 1035,rome,00189, Italy, department of cytology and histology,university of rome la sapienza,ospedale s. andrea,via di grottarossa 1035,rome,00189, Italy, department of cytology and histology,university of rome la sapienza,ospedale s. andrea,via di grottarossa 1035,rome,00189, Italy, department of radiological,oncological and pathological sciences,sapienza university of rome,policlinico umberto i,viale regina elena 324,rome,00161, Italy, department of radiological,oncological and pathological sciences,sapienza university of rome,policlinico umberto i,viale regina elena 324,rome,00161, Italy, clinical experimental oncology department,national cancer research centre,istituto tumori giovanni paolo ii,bari, Italy, unit of medical statistics,biometry and bioinformatics,fondazione irccs istituto nazionale dei tumori,milan, Italy, pathology department,regina elena national cancer institute,via elio chianesi 53,rome,00144, Italy
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Authors
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