MicroRNA-195 suppresses tumor cell proliferation and metastasis by directly targeting BCOX1 in prostate carcinoma

Elucidation of the downstream targets regulated by the metastasis-suppressive mirnas can shed light on the metastatic processes in prostate cancer (pca). we conducted microarray analyses and found that mir-195 was significantly decreased in metastatic pca. low mir-195 expression is an independent prognostic factor for poor biochemical recurrence-free and overall survival. forced expression of mir-195 in pca cells drastically inhibits proliferation,migration and invasion in vitro and inhibits tumor growth and metastasis in vivo. bcox1 is identified as a direct target of mir-195 in pca,and is found to be drastically increased in metastatic pca. bcox1 knockdown phenotypically copies mir-195-induced phenotypes,whereas forced expression of bcox1 reverses the effects of mir-195. collectively,this is the first report unveils that loss of mir-195 expression and thus uncontrolled bcox1 upregulation might drive pca metastasis. © 2015 guo et al.