|
|
|
|
Angiotensin II type 2 receptor promotes apoptosis and inhibits angiogenesis in bladder cancer
|
|
|
|
|
|
|
|
نویسنده
|
pei n. ,mao y. ,wan p. ,chen x. ,li a. ,chen h. ,li j. ,wan r. ,zhang y. ,du h. ,chen b. ,jiang g. ,xia m. ,sumners c. ,hu g. ,gu d. ,li h.
|
|
منبع
|
journal of experimental and clinical cancer research - 2017 - دوره : 36 - شماره : 1
|
|
چکیده
|
Background: bladder cancer (bca) is the ninth most common form of cancer in the world. there is a continuing need not only for improving the accuracy of diagnostic markers but also for the development of new treatment strategies. recent studies have shown that the renin-angiotensin system (ras),which include the angiotensin type 1 (at1r),type 2(at2r),and mas receptors,play an important role in tumorigenesis and may guide us in meeting those needs. results: in this study,we first observed that at1r and mas expression levels were significantly upregulated in bca specimens while at2r was significantly downregulated. viral vector mediated overexpression of at2r induced apoptosis and dramatically suppressed bca cell proliferation in vitro,suggesting a therapeutic effect. investigation into the mechanism revealed that the overexpression of at2r increases the expression levels of caspase-3,caspase-8,and p38 and decreases the expression level of perk. at2r overexpression also leads to upregulation of 2 apoptosis-related genes (bcl2a1,tnfsf25) and downregulation of 8 apoptosis-related genes (casp 6,casp 9,dffa,igf1r,pycard,tnf,tnfrsf21,tnfsf10,naip) in transduced ej cells as determined by pcr array analysis. in vivo,we observed that at2r overexpression caused significant reduction in xenograft tumors sizes by downregulation vegf and induction of apoptosis. conclusions: taken together,the data suggest that at1r,at2r or mas could be used as a diagnostic marker of bca and at2r is a promising novel target gene for bca gene therapy. © 2017 the author(s).
|
|
|
|
|
آدرس
|
department of clinical pathology,first affiliated hospital,jinan university,guangzhou,guangdong,china,school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, department of biomedical engineering,johns university,school of medicine,baltimore, United States, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, department of otolaryngology-head and neck surgery,guangdong no. 2 provincial people's hospital,guangzhou,guangdong, China, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China, department of clinical pathology,first affiliated hospital,jinan university,guangzhou,guangdong, China, department of clinical pathology,first affiliated hospital,jinan university,guangzhou,guangdong, China, department of physiology and functional genomics,university of florida,gainesville,fl, United States, college of animal science and technology,jilin agricultural university,xincheng street no. 2888,changchun,130118, China, department of urology,421 st hospital of pla,no. 350,xinggang rd,haizhu district,guangzhou,guangdong,510318, China, school of laboratory medicine and biotechnology,southern medical university,1023 south shatai road,guangzhou,guangdong,510515, China
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|