|
|
|
|
β-catenin-mediated YAP signaling promotes human glioma growth
|
|
|
|
|
|
|
|
نویسنده
|
wang y. ,pan p. ,wang z. ,zhang y. ,xie p. ,geng d. ,jiang y. ,yu r. ,zhou x.
|
|
منبع
|
journal of experimental and clinical cancer research - 2017 - دوره : 36 - شماره : 1
|
|
چکیده
|
Background: hippo/yap pathway is known to be important for development,growth and organogenesis,and dysregulation of this pathway leads to tumor progression.we and others find that yap is up-regulated in human gliomas and associated with worse prognosis of patients. however,the role and mechanism of yap in glioma progression is largely unknown. methods: the expression of yap in glioma tissues was detected by quantitative polymerase chain reaction (qpcr) and immunoblotting. the effect of yap on glioma progression was examined using cell growth assays and intracranial glioma model. the effect of yap on β-catenin protein level,subcellular location and transcription activity was examined by immunoblotting,immunofluorescence and rt-pcr. results: firstly,knockdown of yap inhibited glioma cell proliferation in vitro and tumor growth in vivo. in addition,yap modulated the protein level,subcellular location and transcription activity of β-catenin via regulating the activity of gsk3β. lastly,β-catenin partially mediated the effect of yap on glioma cell proliferation. conclusion: our findings identify that yap promotes human glioma growth through enhancing wnt/β-catenin signaling. in addition,this study provides a new crosstalk mechanism between hippo/yap and wnt/β-catenin pathways,which suggests a new strategy for human glioma treatment. © 2017 the author(s).
|
|
کلیدواژه
|
Glioma; GSK3β; Nude mice; Proliferation; YAP; β-catenin
|
|
آدرس
|
insititute of nervous system diseases,xuzhou medical university,84 west huai-hai road,xuzhou,jiangsu 221002,china,brain hospital,affiliated hospital of xuzhou medical university,xuzhou,jiangsu, China, graduate school,xuzhou medical university,xuzhou,jiangsu,china,present address: department of neurosurgery,xuzhou cancer hospital,xuzhou,jiangsu, China, graduate school,xuzhou medical university,xuzhou,jiangsu, China, graduate school,xuzhou medical university,xuzhou,jiangsu, China, graduate school,xuzhou medical university,xuzhou,jiangsu, China, graduate school,xuzhou medical university,xuzhou,jiangsu, China, graduate school,xuzhou medical university,xuzhou,jiangsu, China, insititute of nervous system diseases,xuzhou medical university,84 west huai-hai road,xuzhou,jiangsu 221002,china,brain hospital,affiliated hospital of xuzhou medical university,xuzhou,jiangsu,china,jiangsu center for the collaboration and innovation of cancer biotherapy,cancer institute,xuzhou medical university,xuzhou,jiangsu, China, insititute of nervous system diseases,xuzhou medical university,84 west huai-hai road,xuzhou,jiangsu 221002,china,brain hospital,affiliated hospital of xuzhou medical university,xuzhou,jiangsu,china,jiangsu center for the collaboration and innovation of cancer biotherapy,cancer institute,xuzhou medical university,xuzhou,jiangsu, China
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|