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   Synthetic regulatory RNAs selectively suppress the progression of bladder cancer  
   
نویسنده zhuang c. ,huang x. ,zhuang c. ,luo x. ,zhang x. ,cai z. ,gui y.
منبع journal of experimental and clinical cancer research - 2017 - دوره : 36 - شماره : 1
چکیده    The traditional treatment for cancer is lack of specificity and efficacy. modular synthetic regulatory rnas,such as inhibitive rna (irna) and active rna (arna),may overcome these limitations. here,we synthesize a new irna to bind the upstream activating sequence (uas) of a minimal promoter that drives expression of artificial mirnas (amirnas) targeting myc,which represses the binding interaction between uas and gal4 fusion protein (gal4-vp64) in gal4/uas system. the arna driven by a tumor-specific mutant human telomerase reverse transcriptase (htert) promoter is created to interact with irna to expose uas again in bladder cancer. without the arna,mrna and protein levels of myc,cell growth,cell apoptosis and cell migration were no significance in two bladder cancer cell lines,t24 and 5637,and human foreskin fibroblast (hff) cells. the arna significantly inhibited the expression of myc in mrna and protein levels,as well as the proliferation and migration of the cancer cells,but not in hff cells. these results indicated that regulatory rnas selectively controlled the expression of amirnas and ultimately suppress the progression of bladder cancer cells without affecting normal cells. synthetic regulatory rnas might be a selective therapeutic approach for bladder cancer. © 2017 the author(s).
کلیدواژه Artificial miRNA; Bladder cancer; GAL4; Human telomerase reverse transcriptase
آدرس guangdong and shenzhen key laboratory of male reproductive medicine and genetics,institute of urology,peking university shenzhen hospital,shenzhen-peking university,hong kong university of science and technology medical center,shenzhen,518000, China, guangdong and shenzhen key laboratory of male reproductive medicine and genetics,institute of urology,peking university shenzhen hospital,shenzhen-peking university,hong kong university of science and technology medical center,shenzhen,518000, China, guangdong and shenzhen key laboratory of male reproductive medicine and genetics,institute of urology,peking university shenzhen hospital,shenzhen-peking university,hong kong university of science and technology medical center,shenzhen,518000, China, guangdong and shenzhen key laboratory of male reproductive medicine and genetics,institute of urology,peking university shenzhen hospital,shenzhen-peking university,hong kong university of science and technology medical center,shenzhen,518000, China, department of biochemistry and molecular biology,school of basic medical sciences,peking university health science center,beijing,100191, China, guangdong and shenzhen key laboratory of male reproductive medicine and genetics,institute of urology,peking university shenzhen hospital,shenzhen-peking university,hong kong university of science and technology medical center,shenzhen,518000, China, guangdong and shenzhen key laboratory of male reproductive medicine and genetics,institute of urology,peking university shenzhen hospital,shenzhen-peking university,hong kong university of science and technology medical center,shenzhen,518000, China
 
     
   
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